Clustered Regularly Interspaced Short Palindromic

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology is a revolutionary gene-editing tool enabling precise genomic modifications, with research currently focusing on improving the accuracy and efficiency of CRISPR systems like Cas9 and Cas13. This involves developing advanced predictive models, such as deep learning architectures and ensemble methods, to estimate on-target efficacy and minimize off-target effects, as well as employing hierarchical clustering techniques to analyze high-dimensional single-cell data generated by CRISPR screens. The ability to accurately predict and control CRISPR outcomes is crucial for advancing gene therapy, drug discovery, and fundamental biological research.