Light Sheet Fluorescence Microscopy

Light-sheet fluorescence microscopy (LSFM) is a powerful 3D imaging technique offering high-resolution visualization of biological samples with reduced phototoxicity compared to other methods. Current research focuses on overcoming challenges like image artifacts (e.g., stripes, uneven illumination) through advanced computational methods such as graph neural networks and level set algorithms, and improving image quality via techniques like dual-view imaging and compressive sensing. These advancements enable more accurate and efficient 3D reconstruction, particularly for large-scale datasets, facilitating improved analysis of complex biological structures and processes in fields like developmental biology and neuroscience. The integration of LSFM with other imaging modalities, such as X-ray microscopy, further enhances its utility for comprehensive multi-modal analysis.