GATHER: Convergence-Centric Hyper-Entity Retrieval for Zero-Shot Cell-Type Annotation
Authors: Zhonghui Zhang, Feng Jiang, Shaowei Qin, Jiahao Zhao, Min Yang
Abstract
Zero-shot single-cell cell-type annotation aims to determine a cell's type from a given set of expressed genes without any training. Existing knowledge-graph-based RAG approaches retrieve evidence by expanding from source entities and relying on iterative LLM reasoning. However, in this setting each query contains tens to hundreds of genes, where no single gene is decisive and the label emerges only from their collective co-occurrence. Such hyper-entity queries fundamentally challenge local, entity-wise exploration strategies, which reason from individual genes, leading to poor scalability and substantial LLM cost. We propose GATHER (Graph-Aware Traversal with Hyper-Entity Retrieval), a convergence-centric retriever tailored to hyper-entity queries. It performs global multi-source graph traversal and identifies topological convergence points -- nodes jointly reachable from many input genes. These convergence nodes act as high-information hyper-entities that capture entity synergy. By incorporating node- and path-importance scoring, GATHER selects informative evidence entirely without LLM involvement during retrieval. Instantiated on a self-constructed cell-centric biological knowledge graph (VCKG), GATHER outperforms strong KG-RAG baselines (ToG, ToG-2, RoG, PoG) on two datasets (Immune and Lung), achieving the highest exact-match accuracy (27.45% and 59.64%) with only a single LLM call per sample, compared to 2--61 calls for KG-RAG baselines. Our results demonstrate that convergence nodes compress multi-entity signals into compact, high-information evidence that conveys more per item than multi-hop paths, providing an efficient global alternative to local entity-wise reasoning.
Graph-structured retrieval-augmented generation (RAG) systems can improve answer quality on multi-hop questions, but many current systems rely on large language models (LLMs) to extract entities, relations, and summaries during indexing. These calls add token and wall-clock costs that grow with corpus size. We present ContextRAG, a graph RAG system whose graph topology is constructed without LLM-based entity or relation extraction. ContextRAG derives a fuzzy concept graph over chunk embeddings using residual-quantization k-means and Formal Concept Analysis with Lukasiewicz residuated logic. Bridge-like and meet-derived context nodes are induced by soft fuzzy join and meet operations, rather than by LLM-written graph edges. On a 130-task UltraDomain subset, ContextRAG builds its index with 30 LLM calls and 22,073 tokens. In contrast, a local HiRAG reproduction stress test required 870 indexing calls and 3.54M tokens on a 20-task subset before failing during graph construction; linear extrapolation to 130 tasks implies over 23M indexing tokens. ContextRAG obtains 33.6% F1 overall and 36.8% F1 on multi-hop tasks. An activation analysis shows that queries retrieving at least one lattice-derived node in the top five achieve +3.9 percentage points F1 over queries that do not; this association is diagnostic rather than causal.
Retrieval-Augmented Generation (RAG) has emerged as a paradigm for enhancing large language models (LLMs) with external knowledge, yet existing graph-based methods face a fundamental limitation: entity-centric and chunk-centric approaches operate on representations anchored to original text without true knowledge fusion. While entity-centric methods connect logically related content and chunk-centric methods preserve context, both retrieve information separately through similarity search, missing emergent understanding from their synthesis. In this paper, we propose HyGRAG, a hierarchical graph RAG framework that transcends source documents by addressing three core challenges: constructing summaries that genuinely integrate contextual and relational information, leveraging these synthesized representations to access emergent knowledge during retrieval, and efficiently updating hierarchical structures for dynamic corpora. Specifically, we design hierarchical index structures over hybrid graphs with both chunk and entity nodes, then iteratively cluster them and generate LLM-based summaries. Then, we design context and relation-aware retrieval that searches across all abstraction levels while expanding through community membership. Moreover, we enable dynamic knowledge update through attachment-based algorithms with only local re-summarization. Experimental results show that HyGRAG improves the average accuracy of multi-hop reasoning tasks by 9.7%, while maintaining reasonable efficiency.
Graph-based Retrieval-Augmented Generation (GraphRAG) enhances LLMs by structuring corpus into graphs to facilitate multi-hop reasoning. While recent lightweight approaches reduce indexing costs by leveraging Named Entity Recognition (NER), they rely strictly on structural co-occurrence, failing to capture latent semantic connections between disjoint entities. To address this, we propose EHRAG, a lightweight RAG framework that constructs a hypergraph capturing both structure and semantic level relationships, employing a hybrid structural-semantic retrieval mechanism. Specifically, EHRAG constructs structural hyperedges based on sentence-level co-occurrence with lightweight entity extraction and semantic hyperedges by clustering entity text embeddings, ensuring the hypergraph encompasses both structural and semantic information. For retrieval, EHRAG performs a structure-semantic hybrid diffusion with topic-aware scoring and personalized pagerank (PPR) refinement to identify the top-k relevant documents. Experiments on four datasets show that EHRAG outperforms state-of-the-art baselines while maintaining linear indexing complexity and zero token consumption for construction. Code is available at https://github.com/yfsong00/EHRAG.