A Flexible ROI Analysis Tool for Spatially Consistent Vegetation Index Extraction from Turfgrass Imagery

Hyperspectral imaging (HSI) allows researchers to study plant traits non-destructively. By capturing hundreds of narrow spectral bands per pixel, it reveals details about plant biochemistry and stress that standard cameras miss. However, processing this data is often challenging. Many labs still rely on loosely organized collections of lab-specific MATLAB or Python scripts, which makes workflows difficult to share and results difficult to reproduce. MVOS_HSI is an open-source Python library that provides an end-to-end workflow for processing leaf-level HSI data. The software handles everything from calibrating raw ENVI files to detecting and clipping individual leaves based on multiple vegetation indices (NDVI, CIRedEdge and GCI). It also includes tools for data augmentation to create training-time variations for machine learning and utilities to visualize spectral profiles. MVOS_HSI can be used as an importable Python library or run directly from the command line. The code and documentation are available on GitHub. By consolidating these common tasks into a single package, MVOS_HSI helps researchers produce consistent and reproducible results in plant phenotyping

We present a large-scale unmanned aerial vehicle (UAV)-based RGB and multispectral image dataset collected over paddy fields in the Vijayawada region, Andhra Pradesh, India, covering nursery to harvesting stages. We used a 20-megapixel RGB camera and a 5-megapixel four-band multispectral camera capturing red, green, red-edge, and near-infrared bands. Standardised operating procedure (SOP) and checklists were developed to ensure repeatable data acquisition. Our dataset comprises of 42,430 raw images (415 GB) captured over 5 acres with 1 cm/pixel ground sampling distance (GSD) with associated metadata such as GPS coordinates, flight altitude, and environmental conditions. Captured images were validated using Pix4D Fields to generate orthomosaic maps and vegetation index maps, such as normalised difference vegetation index (NDVI) and normalised difference red-edge (NDRE) index. Our dataset is one of the few datasets that provide high-resolution images with rich metadata that cover all growth stages of Indian paddy crops. The dataset is available on IEEE DataPort with DOI, . It can support studies on targeted spraying, disease analysis, and yield estimation.

Accurate weed mapping in cereal fields requires pixel-level segmentation from UAV imagery that remains reliable across fields, seasons, and illumination. Existing multispectral pipelines often depend on thresholded vegetation indices, which are brittle under radiometric drift and mixed crop--weed pixels, or on single-stream CNN and Transformer backbones that ingest stacked bands and indices, where radiance cues and normalized index cues interfere and reduce sensitivity to small weed clusters embedded in crop canopy. We propose VISA, a two-stream segmentation network that decouples these cues and fuses them at native resolution. The radiance stream learns from calibrated five-band reflectance using local residual convolutions, channel recalibration, spatial gating, and skip-connected decoding, which preserve fine textures, row boundaries, and small weed structures that are often weakened after ratio-based index compression. The index stream operates on vegetation-index maps with windowed self-attention to model local structure efficiently, state-space layers to propagate field-scale context without quadratic attention cost, and Slot Attention to form stable region descriptors that improve discrimination of sparse weeds under canopy mixing. To support supervised training and deployment-oriented evaluation, we introduce BAWSeg, a four-year UAV multispectral dataset collected over commercial barley paddocks in Western Australia, providing radiometrically calibrated blue, green, red, red edge, and near-infrared orthomosaics, derived vegetation indices, and dense crop, weed, and other labels with leakage-free block splits. On BAWSeg, VISA achieves 75.6% mIoU and 63.5% weed IoU with 22.8 M parameters, outperforming a multispectral SegFormer-B1 baseline by 1.2 mIoU and 1.9 weed IoU. Under cross-plot and cross-year protocols, VISA maintains 71.2% and 69.2% mIoU, respectively.