Automated identification of Ichneumonoidea wasps via YOLO-based deep learning: Integrating HiresCam for Explainable AI

Declining insect populations make reliable biodiversity monitoring increasingly urgent, yet monitoring of insect biodiversity is hampered by a lack of standardised data and by costly and time-consuming manual identification by expert entomologists. Deep learning-based image classifiers, processing data from automated non-lethal camera traps, have the potential to transform and scale insect biodiversity monitoring. However, challenges remain in acquiring expert-annotated datasets, developing model architectures that generalise well across diverse taxonomic levels and training models on highly imbalanced data. Hierarchical data also benefits from designing models that default to higher-confidence, coarser-level predictions, when uncertain about finer taxonomic levels. In this paper we address these challenges with a deep learning-based hierarchical classification model. First, we present a manually curated, long-tailed dataset of around one million images of insects, extracted from 1,801 camera-trap video recordings and annotated with a five-level, 34-class hierarchy. Further, we adapt a hierarchical classification model architecture to a five-level variable-depth hierarchy, with class-balanced weighting. Our model improves on non-hierarchical classifiers by leveraging biological taxonomy to extract granularity-specific visual features and makes hierarchy-consistent predictions to the deepest taxonomic level that meets a confidence threshold (T = 0.6). Our model achieved a per-level accuracy of 80-99% on test data, across five levels of hierarchy. Furthermore ...

Identifying species in biology among tens of thousands of visually similar taxa while discovering unknown species in open-world environments remains a fundamental challenge in biodiversity research. Current methods treat identification and discovery as separate problems, with classification models assuming closed sets and discovery relying on threshold-based rejection. Here we present DeepTaxon, a retrieval-augmented multimodal framework that unifies species identification and discovery through interpretable reasoning over retrieved visual evidence. Given a query image, DeepTaxon retrieves the top-$k$ candidate species with $n$ exemplar images each from a retrieval index and performs chain-of-thought comparative reasoning. Critically, we redefine discovery as an explicit, retrieval-based decision problem rather than an implicit parametric memory problem. A sample is novel if and only if the retrieval index lacks sufficient evidence for identification, so each retrieval naturally yields a classification or discovery label without manual annotation, thereby providing automatic supervision for both tasks. We train the framework via supervised fine-tuning on synthetic retrieval-augmented data, followed by reinforcement learning on hard samples, converting high-recall retrieval into high-precision decisions that scale to massive taxonomic vocabularies. Extensive experiments on a large-scale in-distribution benchmark and six out-of-distribution datasets demonstrate consistent improvements in both identification and discovery. Ablation studies further reveal effective test-time scaling with candidate count $k$ and exemplar count $n$, strong zero-shot transfer to unseen domains, and consistent performance across retrieval encoders, establishing an interpretable solution for biodiversity research.

Edible insects offer an efficient source of alternative protein, requiring less land, water and emitting less greenhouse gas than conventional livestock. However, their successful integration into the food supply chain demands reliable species authentication to control allergen exposure, prevent adulteration, and meet regulatory standards. Near-infrared spectroscopy provides a rapid analytical tool, but its performance drops when applied to production batches unseen during training due to batch-to-batch variation in spectral measurements. We introduce the Batch-Invariant Spectral Network (BISN), an end-to-end framework that combines a learnable preprocessing module, initialised with Savitzky-Golay filtering, with an entropy-regularised adversarial objective to suppress batch-specific spectral variation. In contrast to Domain-Adversarial Neural Networks, which enforce domain adaptation only after feature extraction, BISN suppress batch-effects before species-specific features are learned. Using 2,700 spectra from three species (Acheta domesticus, Hermetia illucens, and Tenebrio molitor) collected across three independent production batches, BISN achieves a mean leave-one-batch-out accuracy of 0.93 (standard deviation 0.04), outperforming the strongest baseline by four percent. Further insights gained by using explainable AI confirm that model decisions consistently rely on the lipid and protein absorption regions across all folds, connecting predictive performance to known insect biochemistry. BISN addresses both cross-batch robustness and biochemical interpretability for automated insect species authentication under realistic industrial conditions. The source code and dataset are publicly available at https://github.com/majharB/bisn.