Benchmarking Resource-Efficient LLMs for Research Topic Ontology Generation in the Biomedical Field

Ontology learning from text remains challenging despite significant progress in Large Language Models (LLMs), which can hallucinate domain terms, produce inconsistent formats, and favor hierarchical over associative relations. In the LLMs4OL 2026 Challenge, we address both the End-to-End Flagship Task (Task A) and Ontology Extension Reuse Task (Task B) using an offline retrieval-augmented few-shot prompting pipeline. Our system employs Qwen2.5-14B-Instruct with all-MiniLM-L6-v2 for demonstration retrieval, selecting the top-5 examples for Task A and top-2 for Task B. A left-truncated context-windowing strategy preserves task instructions within long prompts. For Task B, generated triples undergo deterministic vocabulary-constrained filtering, retaining triples when at least one endpoint belongs to the sample's closed term/type vocabulary and removing duplicates of the initial ontology. The approach achieves Semantic Graph Similarity of 0.8692, Term-Typing F1 of 0.9200, and Taxonomy Discovery F1 of 0.8540 on Task B, while Task A achieves 0.7416 Semantic Graph Similarity. However, no non-taxonomic relations are extracted, highlighting limitations of closed, taxonomy-oriented relation vocabularies.

The effect of Large Language Model (LLM) scale on ontology learning (OL) performance remains insufficiently characterized. We present a controlled evaluation of 13 models spanning dense and Mixture-of-Experts variants from the Qwen3.5 and Qwen3.6 lineages, together with proprietary GPT release variants, using the OntoLearner retrieval-augmented generation pipeline. All models are evaluated with the same embedding model, retrieval configuration, prompt templates, decoding settings, datasets, and metrics on term typing, taxonomy discovery, and non-taxonomic relationship extraction across four biomedical and materials science and engineering ontologies. Within the dense Qwen3.5 lineage, increasing parameter count primarily improves precision rather than recall, with the largest gains occurring between 9B and 27B parameters. However, the effect of scale is neither monotonic nor uniform across tasks and domains. Dense 27B models outperform substantially larger sparse models on term typing, whereas larger Mixture-of-Experts models achieve the strongest open-weight results on taxonomy discovery. Non-taxonomic relationship extraction remains difficult across model scales, particularly for the Materials Data Science ontology. Performance differences across matched Qwen variants and proprietary GPT releases further indicate that architecture and model lineage can outweigh nominal parameter count. These findings show that model size alone is an insufficient selection criterion for OL and provide empirical guidance for reproducible LLM-assisted ontology engineering.

Despite the success of large language models (LLMs) on general-purpose tasks, their performance in highly specialized domains such as biomedicine remains unsatisfactory. A key limitation is the inability of LLMs to effectively leverage biomedical tools, which clinical experts and biomedical researchers rely on extensively in daily workflows. While recent general-domain tool-calling datasets have substantially improved the capabilities of LLM agents, existing efforts in the biomedical domain largely rely on in-context learning and restrict models to a small set of tools. To address this gap, we introduce BioTool, a comprehensive biomedical tool-calling dataset designed for fine-tuning LLMs. BioTool comprises 34 frequently used tools collected from the NCBI, Ensembl, and UniProt databases, along with 7,040 high-quality, human-verified query-API call pairs spanning variation, genomics, proteomics, evolution, and general biology. Fine-tuning a 4-billion-parameter LLM on BioTool yields substantial improvements in biomedical tool-calling performance, outperforming cutting-edge commercial LLMs such as GPT-5.1. Furthermore, human expert evaluations demonstrate that integrating a BioTool-fine-tuned tool caller significantly improves downstream answer quality compared to the same LLM without tool usage, highlighting the effectiveness of BioTool in enhancing the biomedical capabilities of LLMs. The full dataset and evaluation code are available at https://github.com/gxx27/BioTool