Training Set Synthesis for Bioacoustic Denoising: A Case Study With Mice
Authors: Reyhaneh Abbasi, Peter Balazs, Vincent Lostanlen, Clara Hollomey, Dustin J. Penn, Sarah M. Zala, Nicki Holighaus
Abstract
Bioacoustic recordings are often degraded by ambient noise, which complicates the analysis of weak or noise-overlapped vocalizations. Convolutional neural networks, particularly U-Net architectures, have shown a strong denoising performance in speech and music processing. However, their direct application to bioacoustic signals is limited by the scarcity of clean training data. To address this issue, we propose a training set synthesis approach and develop a supervised denoising model that predicts a complex ratio mask in the time-frequency domain. The model leverages ridges, or frequency contours, that represent the fundamental frequency together with one or more harmonic partial components of vocalizations. These ridges are used both for the synthesis of training sets and to design a loss function that assigns higher weights to the ridge regions (ridge-guided loss function). This weighting step helps the network better preserve vocalization details during denoising. As a case study, we evaluate our approach using ultrasonic vocalizations (USVs) recordings of house mice, which are widely studied in behavioral biology and neuroscience. In actual field recordings, the proposed method enhances fundamental and harmonic partial ridge tracking compared to our previous signal-processing approach. In addition, a classifier trained on denoised data improves USV classification on out-of-sample, noisy recordings from wild and domesticated mice compared to classifiers trained on noisy recordings. Our proposed method also substantially improves the scale-invariant signal-to-distortion ratio on synthetic testing data across a wide range of input signal-to-noise ratios. Although we focus on USVs, the proposed approach should be broadly applicable to other bioacoustic signals with trackable ridges, and thus enables ridgebased training set synthesis and denoising.
Most work in audio enhancement targets human speech, while bioacoustics is less studied due to noisy recordings and the distinct traits of animal sounds. To fill this gap, we adapt speech enhancement methods and build BioSEN, a model made for bioacoustic signals. BioSEN has three modules: a multi-scale dual-axis attention unit for time-frequency feature extraction, a bio-harmonic multi-scale enhancement unit for capturing harmonic structures, and an energy-adaptive gating connection unit that uses frequency weights to keep vocalizations from being removed as noise. Tests on three bioacoustic datasets show that BioSEN matches or exceeds state-of-the-art speech enhancement models while using far less computation. These results show BioSEN's strength for bioacoustic audio enhancement and its promise for biodiversity monitoring and conservation.
Bioacoustic recognition requires fine-grained acoustic understanding to distinguish similar-sounding species. However, many large-scale data repositories such as iNaturalist are weakly annotated, often with only a single positive species label per recording, making supervised learning particularly challenging. Inspired by advances in computer vision, recent approaches have shifted toward self-supervised learning to capture the underlying structure of audio without relying on exhaustive annotations. In particular, masked autoencoders (MAE) have shown strong transferability on massive audio corpora, yet their effectiveness in more modest bioacoustic settings remains underexplored. In this work, we conduct a systematic study of MAE pretraining for species classification on iNatSounds, analyzing the impacts of pretraining data scale, domain specificity, data curation, and transfer strategies. Consistent with prior work, we find that models pretrained on diverse general audio data achieve the best transfer performance on iNatSounds. Contrary to observations from large-scale audio benchmarks, we find that (1) additional masked reconstruction pretraining on domain-specific data provides limited benefits and may even degrade performance relative to off-the-shelf models, and (2) selective data filtering offers a negligible advantage when the overall data scale is limited. Our results indicate that, in moderate-sized fine-grained bioacoustic settings, pretraining scale dominates objective design. These findings further clarify when MAE-based pretraining is effective and provide practical guidance for model selection under limited supervision.
Bioacoustic data from Passive Acoustic Monitoring (PAM) generates large datasets where obtaining detailed auditing and labelling is often impractical, resulting in weak annotations (e.g., presence/absence of species over several minutes of recording). In order to effectively capture the complex temporal patterns and key features of long audio segments, we propose a framework comprising dataset standardisation, feature extraction, and classification via Temporal Convolutional Networks (TCN). This approach eliminates the necessity for setting heuristic decision rules or creating time-consuming strong labels. To demonstrate the effectiveness of our approach, we use sperm whale (\textit{Physeter macrocephalus}) click trains in 4-minute recordings as a case study, from a dataset comprising diverse sources and deployment conditions to maximise generalisability. Our TCN classifiers achieve recall rates exceeding 0.83 at a 0.13 false positive rate, comparable to agreement rates between expert annotators. We compare two methods of feature extraction, Variational AutoEncoders (VAEs) and traditional handpicking of features, and found them to yield similar performance results, with the VAE-based classifiers seeing a more stable performance across datasets and recording conditions. These results offer a way forward in leveraging numerous existing annotated bioacoustic datasets to train automatic classification models, effectively overcoming previous limitations associated with weak labels.
Laia Garrobé Fonollosa, Douglas Gillespie, Lina Stankovic +2