Acquisition Geometry-Assisted Whole-Group Localization of X-ray Fluorescence Maps in Optical Microscopy Images

Registering images acquired with different microscopy modalities is essential for relating complementary measurements of the same specimen. In correlative X-ray fluorescence (XRF) and optical microscopy, the XRF map often covers only a small region of an optical image acquired from the same or an adjacent tissue section. Field-of-view (FOV) localization is necessary but can be difficult when appearance and structure differ across modalities. Here we evaluate training-free vision language model (VLM) localization on two datasets representing same-section high-correspondence and adjacent-section low-correspondence imaging. We test unconstrained and metadata-constrained search and compare VLMs with geometric controls, classical template matching, and two alternative training-free approaches (DINOv2 and multiGradICON). Direct VLM prompting produced content-dependent spatial signals but was not reliable alone. Classical matching was most accurate when cross-modal structure was preserved but failed in the low-correspondence collection. A proposal-and-verify workflow used repeated VLM predictions as candidates and image-based similarity to select the final location. This workflow recovered useful localization in the low-correspondence regime.

Universal object localization in X-ray security inspection is critical for automated threat detection in safety-critical venues. However, unlike everyday RGB images that dominate web-scale visual data, X-ray scans exhibit distinct color patterns, ambiguous boundaries, and compositional structures caused by volumetric superposition. These gaps hinder the direct zero-shot transfer of dense perception foundation models trained on web-scale RGB data. Moreover, annotated X-ray data is scarce and requires expert labeling, limiting both the training of generalizable X-ray native models and the adaptation of RGB foundation models for X-ray data via fine-tuning. Given these challenges, the bright promise of highly generalizable perception models, enabled by data scaling laws in the RGB domain, remains largely out of reach for X-ray inspection. To this end, we introduce LAO-X, a self-supervised adaptation framework that Locates Any Object in X-ray scans using diverse synthesized image--annotation pairs with granularity-aware supervision. LAO-X first designs a saliency-guided X-ray object mining module to separate diverse object instances, which are then used for physics-guided synthesis in the absorbance domain. LAO-X further incorporates an occlusion-controlled curriculum strategy to fine-tune a Segment Anything Model 2 (SAM2) localizer, progressively adapting it to X-ray scans with increasing object counts and overlap levels. Experiments on six X-ray benchmarks show that LAO-X substantially improves category-agnostic localization, achieving 2% to 23% mAP gains over SAM2 and X-ray specific baselines in heavily cluttered scenarios, entirely without human-annotated labels.

X-ray fluorescence (XRF) spectroscopy is a key modality for material analysis in cultural heritage. However, automated learning from XRF spectra remains challenging: XRF spectra are complex one-dimensional signals composed of sharp elemental peaks, broader structures, and background variations that are not taken into account by existing learning-based models. This paper introduces XRFormer, a transformer architecture tailored to XRF spectra through a multiscale convolutional tokenizer that injects locality and multi-resolution inductive biases before global self-attention. The tokenizer progressively reduces spectral resolution while increasing embedding dimensionality, and the resulting token sequence is processed by a standard transformer encoder. We further investigate self-supervised pretraining for XRF representation learning using Masked Spectral modeling (MSM) and a physics-informed Peak Presence Prediction (PPP) objective. Experiments on the Pigments Checker STANDARD v.5 dataset for pigment identification and unmixing show that XRFormer consistently outperforms ViT, SpectralFormer (with and without CAF), and a 1D-CNN baseline for pigment identification. For pigment unmixing, XRFormer achieves robust abundance estimation while maintaining significantly higher parameter efficiency than SpectralFormer, operating at a lower token resolution (128 vs. 512 tokens) and with less than half the number of parameters (1.5M vs. 3.37M). MSM yields consistent gains across both tasks, while PPP further enhances performance for both identification and unmixing when tuned with an appropriate peak prominence. These results highlight multiscale, modality-aware tokenization as an effective and parameter efficient foundation for transformer-based XRF modeling under data-limited conditions. A GitHub repository is provided at https://github.com/sofiane1010/XRFormer.