A Non-Linear Neuron Based Detection of Isolated Pixels in Binary and Grayscale Images using Contrast Sensitive Receptive Fields

Reconstruction-based methods are a cornerstone of unsupervised image anomaly detection, but they remain vulnerable to \emph{outlier leakage}, where standard mean squared error (MSE) loss drives the model to faithfully reconstruct anomalous patterns. We propose a Non-linear Reconstruction Loss that applies a sigmoid-based squashing function to suppress high-magnitude features, preventing outliers from dominating optimization while preserving sensitivity to normal patterns. In addition, we introduce a statistical calibration scheme that selects the scaling factor $k$ from the confidence interval (CI) of the normal feature distribution, enabling data-driven control of the suppression strength. Our approach achieves competitive or superior anomaly detection performance compared to state-of-the-art methods, reaching 99.0% Image-AUROC and 97.3% Pixel-AUROC on MVTec-AD, and 95.3% Image-AUROC and 99.0% Pixel-AUROC on VisA. These results indicate that non-linear gradient suppression is an effective mechanism for mitigating outlier leakage and improving anomaly localization in unified industrial inspection settings. The implementation is available at https://github.com/mintii13/Statistical-Non-linear-Reconstruction-Loss.git.

Detecting subtle visual anomalies in images remains challenging, particularly when only normal samples are available a priori. Such unsupervised anomaly detection is typically solved by measuring feature similarity of a query patch to a memory of normal patches. However, similarity alone does not reveal how strongly a query patch violates the structure of the normal feature manifold. We propose a training-free Laplacian graph energy optimization formulation, named ANoCo that scores Anomaly by the cost of Non-Conformity of a query patch to align with a fixed normal manifold. For each query patch, we construct a bipartite query to normal graph weighted by cosine affinity, explicitly removing query-query and normal-normal edges to prevent evidence dilution. We formulate anomaly scoring as a convex Laplacian energy with anchored normal nodes, and solve in closed form. In particular, we do not use the optimized features themselves-the anomaly score is the magnitude of the update required to satisfy normality constraints, reframing the graph Laplacian as a non-conformity operator rather than a smoothing prior. The proposed method introduces no learnable parameters, message passing, or sampling, and has complexity comparable to a single linear solve. Across standard benchmarks, it delivers strong image-level AUROC, stable localization maps, and improved robustness over prior methods, demonstrating the effectiveness of using optimization-induced feature drift as anomaly measure.

Drug-induced toxicity is a leading cause of preclinical and early-clinical failure, making early detection critical. Histopathology is the gold standard for toxicity assessment but relies on expert pathologists, creating a bottleneck for large-scale screening. We introduce an AI-based anomaly detection framework for whole-slide images (WSIs) of rodent liver that identifies healthy tissue and known pathologies (anomalies) and flags samples without training data as out-of-distribution (OOD). We evaluate OOD detection on two held-out categories: apoptosis (single-cell, near-OOD) and staining/processing artifacts (heterogeneous, far-OOD). We build a novel pixelwise-annotated dataset and fine-tune a pre-trained Vision Transformer (DINOv2) via Low-Rank Adaptation (LoRA) for segmentation, then use the Mahalanobis distance for OOD detection with class-specific thresholds. Optimizing the false positive rate subject to a predefined constraint on the false negative rate yields only 0.16% of pathological tissue classified as healthy and 0.35% of healthy tissue classified as pathological. Our false negative rate does not penalise cross-type errors, reflecting the safety-first objective of never overlooking a lesion; under the stricter correct-class criterion our method assigns 93.93% of ID and 89.38% of OOD findings to their own class. The study demonstrates technical feasibility of pixel-level anomaly detection for mouse liver histopathology, indicating possible applications in improving preclinical workflows and drug development efficiency.