Toward Markerless Video-based Tremor Analysis: Objective Quantification of Pathological Tremor in Mouse Preclinical Models

Tremor is a common movement disorder associated with conditions like Parkinson's disease and Essential tremor, traditionally diagnosed through expert clinician assessment. Current automated detection methods rely on frequency-domain features informed by clinical expertise. In this work, we present an explainable, two-stage hierarchical framework for tremor detection in the time domain that learns tremor patterns directly from 3D kinematic marker time-series data across entire tremor-provoking trials. Our framework combined a deep convolutional and long short-term memory network to learn tremor representations from short, discrete, non-overlapping time segments of kinematic time series data from trials, which are then processed by a vision transformer that models their long-term temporal dynamics of time segment features for trial (session) level classification. Evaluated across nine body parts, the framework achieved F1-scores of 0.594 - 0.947 depending on body parts (average: 0.765), falling short of the frequency-domain state-of-the-art performance (0.909) while requiring minimal preprocessing. Attention weights and gradient-based class activation maps (Grad-CAM) identified time-domain features of tremor across body parts. This proof of concept demonstrated the feasibility of data-driven time-domain modeling for tremor detection across anatomically diverse body parts, while reducing reliance on expert-engineered spectral features and providing posthoc interpretability of temporal and anatomical patterns of tremor.

Motor impairments, including tremor, bradykinesia, gait abnormalities, and postural instability, are common across many neurological and movement-related conditions. Conventional clinical assessments are often intermittent and may fail to capture subtle temporal variations in motor behavior. While wearable IMUs and third-person video have shown promise for objective motor assessment, third-person recordings raise privacy concerns and require constrained acquisition setups. In contrast, egocentric vision provides a more naturalistic and privacyaware alternative. In this work, we introduce EgoInertia-MI, a multimodal benchmark dataset combining synchronized egocentric video and wearable IMU signals for motor impairment analysis. The dataset contains 19 upper- and lower-body activities performed by healthy volunteers simulating varying levels of motor impairment severity levels: no impairment, mild impairment, and severe impairment. We establish two benchmark tasks: action recognition and motor impairment severity estimation, and evaluate multiple unimodal and multimodal baselines. Experimental results show that egocentric video provides strong cues for motor impairment assessment, while multimodal fusion achieves the best overall performance, reaching 0.78 Macro-F1 for severity estimation and 0.93 Macro-F1 for action recognition. These findings highlight the potential of combining egocentric vision and wearable sensing for ecologically valid and privacy-aware motor assessment. Code and data are available at:https://fatemah-alh.github.io/EgoInertia-MI-Page/.

Continuous physiological monitoring is essential for linking behavior, internal state, and neural activity in neuroscience and translational research. In common marmosets, a key nonhuman primate model for social neuroscience, respiratory rate provides a basic readout of arousal, stress, and physiological regulation. Contact sensors can provide accurate measurements, but they add preparation burden and may interfere with head-free behavioral protocols. Thermal video offers a noncontact alternative by capturing breathing-related temperature changes around the nose and mouth. However, in head-free marmosets, these cues are weak, localized, and easily corrupted by head motion, pose changes, and fur. Here we show that this setting exposes a broader challenge in video-based physiological sensing: visually reliable tracking does not necessarily imply physiologically valid measurement. Using synchronized thermal video and contact respiration recordings, we show that marmoset respiration is recoverable from thermal video but strongly ROI-sensitive. Under head-free motion, visually plausible nasal tracking can still produce traces inconsistent with contact respiration, demonstrating that tracking is not sensing. We therefore estimate respiratory rate from learned temporal signals extracted from thermal clips and introduce Selective Temporal Attenuation Regularization (STAR). STAR selectively attenuates high-motion thermal discontinuities through local temporal imputation during training, regularizing models toward respiration-consistent dynamics while leaving inference unchanged on raw video. Across multiple deep-learning backbones, STAR improves head-free estimation, reaching 2.23 bpm MAE compared with 20.76 bpm for tracked ROI analysis.