2-Dimensional spectral gating for denoising bioacoustics recordings

Most work in audio enhancement targets human speech, while bioacoustics is less studied due to noisy recordings and the distinct traits of animal sounds. To fill this gap, we adapt speech enhancement methods and build BioSEN, a model made for bioacoustic signals. BioSEN has three modules: a multi-scale dual-axis attention unit for time-frequency feature extraction, a bio-harmonic multi-scale enhancement unit for capturing harmonic structures, and an energy-adaptive gating connection unit that uses frequency weights to keep vocalizations from being removed as noise. Tests on three bioacoustic datasets show that BioSEN matches or exceeds state-of-the-art speech enhancement models while using far less computation. These results show BioSEN's strength for bioacoustic audio enhancement and its promise for biodiversity monitoring and conservation.

Bioacoustic recordings are often degraded by ambient noise, which complicates the analysis of weak or noise-overlapped vocalizations. Convolutional neural networks, particularly U-Net architectures, have shown a strong denoising performance in speech and music processing. However, their direct application to bioacoustic signals is limited by the scarcity of clean training data. To address this issue, we propose a training set synthesis approach and develop a supervised denoising model that predicts a complex ratio mask in the time-frequency domain. The model leverages ridges, or frequency contours, that represent the fundamental frequency together with one or more harmonic partial components of vocalizations. These ridges are used both for the synthesis of training sets and to design a loss function that assigns higher weights to the ridge regions (ridge-guided loss function). This weighting step helps the network better preserve vocalization details during denoising. As a case study, we evaluate our approach using ultrasonic vocalizations (USVs) recordings of house mice, which are widely studied in behavioral biology and neuroscience. In actual field recordings, the proposed method enhances fundamental and harmonic partial ridge tracking compared to our previous signal-processing approach. In addition, a classifier trained on denoised data improves USV classification on out-of-sample, noisy recordings from wild and domesticated mice compared to classifiers trained on noisy recordings. Our proposed method also substantially improves the scale-invariant signal-to-distortion ratio on synthetic testing data across a wide range of input signal-to-noise ratios. Although we focus on USVs, the proposed approach should be broadly applicable to other bioacoustic signals with trackable ridges, and thus enables ridgebased training set synthesis and denoising.

Pretrained audio embeddings are standard in bioacoustics, yet little is known about which acoustic features these models encode, nor which are useful for a given task. This hinders transparency and limits extension to rare species or data-scarce domains. Here we reveal which speech-like features are encoded in bioacoustic representations. Using the 88~eGeMAPS features across six taxonomic groups, we apply linear and nonlinear regression probes to quantify which acoustic properties each model captures. Results confirm a ``no free lunch'' pattern: no single model captures the full feature space. A concatenated embedding achieves the highest performance, suggesting complementary acoustic space coverage across models. Loudness features are best encoded ($R^2 = 0.76$) while F0 is hardest to recover ($R^2 = 0.33$). By cross-referencing recoverability with per-species feature salience (NMI), we derive data-driven model selection guidance for bioacoustics.