Organizations: University of Carthage, Engineering School of Statistics and Information Analysis, Tunis, Tunisia. · University of Tunis-El-Manar, Rabies Laboratory, Institut Pasteur de Tunis, Tunis, Tunisia. · World Health Organization, Country Office Tunisia. · University of Tunis-El-Manar, BIMS Laboratory (LR16IPT06), Institut Pasteur de Tunis, Tunis, Tunisia.
Rabies remains a major public health concern across many African and Asian countries, where accurate diagnosis is critical for effective epidemiological surveillance. The gold standard diagnostic methods rely heavily on fluorescence microscopy, necessitating skilled laboratory personnel for the accurate interpretation of results. Such expertise is often scarce, particularly in regions with low annual sample volumes. This paper presents an automated, AI-driven diagnostic system designed to address these challenges. We developed a robust pipeline utilizing fluorescent image analysis through transfer learning with four deep learning architectures: EfficientNetB0, EfficientNetB2, VGG16, and Vision Transformer (ViTB16). Three distinct data augmentation strategies were evaluated to enhance model generalization on a dataset of 155 microscopic images (123 positive and 32 negative). Our results demonstrate that TrivialAugmentWide was the most effective augmentation technique, as it preserved critical fluorescent patterns while improving model robustness. The EfficientNetB0 model, utilizing Geometric & Color augmentation and selected through stratified 3fold cross-validation, achieved optimal classification performance on cropped images. Despite constraints posed by class imbalance and a limited dataset size, this work confirms the viability of deep learning for automating rabies diagnosis. The proposed method enables fast and reliable detection with significant potential for further optimization. An online tool was deployed to facilitate practical access, establishing a framework for future medical imaging applications. This research underscores the potential of optimized deep learning models to transform rabies diagnostics and improve public health outcomes.
In this paper, we examine the difficulties of using standard techniques for medical image classification due to long-tailed distributions (wherein rarer conditions have very few samples) resulting in bias towards diagnosing common diseases and away from rarer diseases. We then discuss and implement deep learning models with techniques such as augmentation to minimize error, especially from rarer diseases. We evaluate various different models with AP, F1 score, AUROC, and loss (all on the validation set). We conclude with the promising results from our best model, and potential applications in the healthcare space.
Infectious bovine pinkeye is a contagious ocular disease that adversely affects cattle health, welfare, and agricultural productivity. Conventional diagnosis relies primarily on clinical observation, which can be subjective, time-consuming, and difficult to implement efficiently in large herds or remote settings. This study evaluated and compared You Only Look Once (YOLO) v11 and YOLOv26 for automated bovine pinkeye classification and investigated the effects of class-balancing strategies on model performance. Five variants (n, s, m, l, and x) of each architecture were trained and evaluated using the original imbalanced dataset, Random Minority Oversampling (RMO), and an adapted Synthetic Minority Oversampling Technique (SMOTE). Both YOLOv11 and YOLOv26 demonstrated strong classification performance, although the effects of class balancing varied across model variants. For YOLOv11, RMO-s achieved an accuracy of 0.99, a macro F1-score of 0.98, and a true positive rate (TPR) of 1.00, with no false-negative classifications. RMO-m also achieved a TPR of 1.00 with no false negatives. For YOLOv26, the original l, RMO-m, and RMO-l variants each achieved an accuracy of 0.99 and a macro F1-score of 0.98, with RMO-l attaining a TPR of 1.00 and no false negatives. Overall, RMO generally provided greater improvements in minority-class detection than adapted SMOTE, whereas the strong performance of the original YOLOv26-l demonstrates that oversampling was not necessary for all model variants. These findings demonstrate the potential of YOLOv11 and YOLOv26 for automated detection of bovine pinkeye and support further evaluation for livestock health monitoring.
Michael Abalo, Jameson Brennan, Hossein Moradi Rekabdarkolaee
Malaria remains a leading cause of mortality in sub-Saharan Africa, where scarce diagnostic infrastructure makes timely, accurate diagnosis particularly challenging. While deep learning offers a compelling path toward automated malaria screening, clinical adoption is hindered by computational cost and opacity in decision-making. This work benchmarks four deep learning models spanning a wide range of designed design architectures and model capacities on the NLM-Malaria dataset, jointly evaluating predictive performance, robustness, and post-hoc explainability. We find that lightweight, efficient-by-design models match their heavier counterparts in predictive performance, and the Friedman test confirms no statistically significant performance differences. CAM-based XAI methods consistently localize diagnostically relevant regions, while fine-grained attribution methods produce less targeted explanations, particularly with heavier backbones. Robustness evaluation under three types of image corruption further reveals that model confidence degrades faster than accuracy, providing a practical signal for human review. However, no XAI method is robust to corruption, with explanation reliability degrading at noise levels plausible in clinical practice, even when predictions remain accurate. These findings support the deployment of lightweight architectures for malaria diagnosis in resource-constrained settings, while highlighting the vulnerability of post-hoc explanations as an important consideration for responsible clinical deployment.