Multispectral Blind Image Super-Resolution for Standing Dead Tree Segmentation

Detecting dead trees in aerial imagery is vital for assessing forest health, especially as tree mortality increases globally due to climate change, but domain variability and scarce labeled data often limit model generalization. This study advances the TreeMort-1T-UNet (Tree Mortality 1-Task U-Net) model, initially trained on Finnish aerial imagery (source domain), by applying knowledge distillation (KD) to adapt it to various target domains, including Polish, German, and Estonian datasets representing diverse forest types. We assess four KD variants: Basic, Self, Feature-level, and Ensemble, against a fine-tuning baseline, using Mean Tree IoU, Instance F1-score, Instance Precision, and Mean Centroid Error as key metrics, alongside representational analyses (e.g., cosine similarity, CKA, SSIM, t-SNE, and linear probing) for domain invariance. Feature-level KD outperforms others, yielding a Mean Tree IoU of 0.106, Instance F1-score of 0.63, Instance Precision of 0.55, and Mean Centroid Error of 3.039 on the Polish dataset, with robust precision across other target domains (e.g., 0.15 on Finnish, 0.67 on Polish, 0.60 on German, 0.59 on Estonian). It excels in low-data scenarios with fewer false positives and shows superior representational invariance (e.g., higher deep-layer CKA/SSIM, better domain mixing in t-SNE, and linear probing AUC of 0.95), making it ideal for precision-critical forestry applications. Additional ablation studies confirm that key components like feature alignment enhance its performance balance across metrics. Our findings demonstrate KD's potential to enhance transfer learning in remote sensing, offering a scalable, domain-robust tool for ecological monitoring and sustainable forest management.

Forests worldwide are increasingly threatened by climate change and disturbances such as fire, pests, and pathogens, creating an urgent need for scalable monitoring of tree cover and tree mortality. Aerial imagery from drones and aircraft is a key data source for detailed and large-scale mapping of tree crowns and mortality. However, related progress is limited by the lack of globally representative, harmonized datasets for joint segmentation of tree cover and mortality. We introduce two novel, open, machine-learning-ready datasets to enable joint segmentation of tree cover and tree mortality from centimeter-scale aerial imagery for the first time at global scales. With DTE-aerial-train, we provide a training dataset comprising 385K image patches of size 1024x1024 pixels, with resolutions ranging from 2.5 to 20 cm. It includes multi-class expert-annotated and -audited pseudo-labels for tree cover and mortality. With DTE-aerial-bench, we provide a geographically balanced benchmark test set of 25 globally distributed orthoimages totaling 525 patches with high-quality expert annotations for both tree cover and mortality. Both the training and benchmark datasets span tropical, temperate, boreal, and dryland biomes and cover a wide range of forest structures and mortality patterns. Using the benchmark test set for evaluation, we establish strong reference baselines that improve mortality segmentation across all biomes and scales with significant gains in challenging regions, such as boreal forests, where the F1 score increases from 0.40 to 0.58 with around 45% relative improvement. All data, models, and code will be publicly released under permissive open-source licenses. An interactive visualization of the benchmark dataset is available at deadtrees.earth/releases/dte-aerial-bench.

Hyperspectral imaging is a powerful bioimaging tool which can uncover novel insights, thanks to its sensitivity to the intrinsic properties of materials. However, this enhanced contrast comes at the cost of system complexity, constrained by an inherent trade-off between spatial, spectral, and temporal resolution. To overcome this limitation, we present a self-supervised deep learning-based approach that restores and enhances pixel resolution post-acquisition without requiring external training data beyond the images to be restored. Fine-tuned using metrics aligned with the imaging model, our physics-aware method achieves a 16$\times$ pixel super-resolution enhancement and a 12$\times$ imaging speedup without the need of additional training data for transfer learning. Applied to both synthetic and experimental data from five different sample types, including healthy and diseased tissues, we demonstrate that the model preserves biological integrity, as we did not detect systematic loss of biological features or biologically consequential hallucinations in tested datasets. We also concretely demonstrate the model's ability to reveal disease-associated metabolic changes that would otherwise remain undetectable. Furthermore, we provide physical insights into the model's inner workings, paving the way for future refinements that could potentially reveal novel high resolution features in an explainable manner. All methods are available as open-source software on GitHub.