Morphology-Aware Implicit Super-Resolution Network for Pathological Images
Authors: Jiaming Liang, QiHui Han, Haolin Chen, Chengxin Ye, Jiawen Liu, Jiazhou Chen, Xiaoqi Sheng, Hongmin Cai
Organizations: School of Computer Science and Engineering, South China University of Technology, Guangzhou, China · School of Software Engineering, South China University of Technology, Guangzhou, China · School of Future Technology, South China University of Technology, Guangzhou, China · School of Computer Science and Technology, Guangdong University of Technology, Guangzhou, China
Abstract
Accurate diagnosis in Digital Pathology (DP) relies on high-resolution whole-slide images, yet clinical deployment is often limited by hardware costs. Super-Resolution (SR) offers a promising alternative by computationally enhancing low-resolution acquisitions. However, existing SR methods frequently struggle to preserve fine-grained cellular morphology, leading to texture oversmoothing and blurred structural boundaries under complex tissue variability. To address this issue, we propose Morph-ISR, a morphology-aware implicit super-resolution framework for DP that restores diagnostically relevant details with sub-pixel precision. Morph-ISR reformulates SR as a continuous coordinate-based reconstruction problem and integrates an Implicit Position-aware Kernel Generator (IPKG) to adaptively model spatially varying tissue morphology. To further enhance structural fidelity, a Morphological Fidelity Prior (MFP) is introduced, leveraging semantic guidance from a pre-trained cell segmentation network to enforce boundary-preserving and region-aware reconstruction, thereby improving the representation of critical cellular boundaries and nuclear textures. Experiments on TCGA and SurGen datasets show that Morph-ISR achieves the best LPIPS and ST-LPIPS among the evaluated methods, reducing them by up to 38.37% and 39.55%, respectively, over the second-best methods while maintaining strong PSNR and SSIM. These results demonstrate superior preservation of diagnostically relevant cellular boundaries and nuclear textures, while compact parameterization and high throughput support efficient edge deployment. Code and trained models will be released upon publication.
Digital pathology relies on high-resolution whole slide images for accurate diagnosis, yet limitations in imaging devices, storage, and transmission often make lower-resolution pathology images more common in clinical workflows. Current super-resolution techniques often tend to smooth diagnostically relevant morphology, leading to over-smoothed textures and semantic drift that compromise downstream clinical interpretation. To this end, we develop the Structural Semantic Synergy Diffusion Model (S3-Diff), a diffusion framework for high-fidelity super-resolution of pathological images. The core of S3-Diff is Specimen-aware Structural Anchoring (SSA), which combines prognosis-aware tissue support extracted by a fixed SAM with LR-HR gradient discrepancies to generate a specimen-specific structural anchor to preserve pathological morphology. Concurrently, we introduce Structure-guided Semantic Fidelity Tuning (SSFT) to adapt DINOv3 representations using SSA-derived structural supervision. SSFT combines the adapted semantic energy with LR-derived edge and grayscale cues. The resulting control guides denoising to suppress stochastic artifacts and maintain structural consistency. Extensive experimental results demonstrate that S3-Diff consistently outperforms state-of-the-art methods in both reconstruction quality and downstream survival analysis performance. The source code will be made public.
Vision Transformers (ViTs) and their hierarchical variants have achieved strong performance in Computational Pathology (CPath). However, most are pre-trained on single-resolution Whole Slide Images (WSIs), limiting their generalization across arbitrary resolutions. Gigapixel WSIs inherently contain diagnostic patterns at multiple scales, including cellular morphologies, tissue architectures, and global context, mirroring how expert pathologists examine WSIs. We introduce Multi-Resolution Pyramid Transformer (MRPT), a model that hierarchically aggregates multi-resolution information from cellular to tissue and WSI levels. MRPT employs a biologically meaningful Consecutive Cross-Resolution Attention (CCRA) mechanism to capture scale-independent interactions and enforces multi-resolution semantic consistency by aligning embeddings across resolutions, yielding robust and generalizable WSI representations. Pre-trained in a multi-resolution self-supervised manner on 624M patches, 2.4M regions, and 36K WSIs, MRPT learns rich coarse-to-fine histopathology features. Extensive experiments on 34 diverse datasets show that MRPT surpasses recent foundation models and Multimodal Large Language Models (MLLMs) in cancer subtype classification, tissue phenotyping, and Visual Question Answering (VQA) for WSI understanding.
Basit Alawode, Moshira Ali Abdalla, Dwarikanath Mahapatra +2
Super-resolution can make inspection images appear sharper without preserving the evidence needed to detect a defect. We study this failure mode with a benchmark that separates reconstruction from detection and evaluates both at a predeclared low false-positive rate. Ten end-to-end repetitions combine independently generated line/space and contact-hole images with model training, calibration, clean controls, weak defects, and a held-out defect morphology. Every reconstruction is scored by the same local residual detector, while direct and jointly trained detectors form a separate comparison track. Reconstruction fidelity and inspection utility diverge: the two learned reconstruction models attain the highest structural similarity yet detect fewer defect pixels than bicubic interpolation in every paired repetition. A direct DeepLabV3 detector reaches 0.1984±0.0385 recall at 0.000174±0.000084 false-positive rate and satisfies the held-out feasibility criterion in all ten repetitions. An illustrative joint model, DPU-WaferSR, passes independent clean calibration but exceeds the held-out limit in all ten repetitions, demonstrating that calibration success does not guarantee transfer. Weak-defect recall remains near zero for every feasible method. Applying the unchanged policies to 4,591 public Carinthia-S masks further reveals large method-dependent shifts on real SEM texture. These results support a simple conclusion: super-resolution for inspection should be judged by preserved task evidence and operating-point transfer, not reconstruction quality alone.