Detection of Virus and Small Cell Patches in Foci Images Using Switchable Convolution and Feature Pyramid Networks

Pathology foundation models (FMs) are models trained on vast amounts of typically unlabeled data and have been shown to yield regularized latent spaces that can be used effectively in downstream classification tasks. This is also true for the classification of mitotic figures vs. other cells. However, it is so far unclear if the latent space of current FMs provides features that are discriminant and spatially suitably resolved to also serve as a backbone for dense object detection paradigms. In this work, we investigate this question for common current pathology FMs (UNI, UNI2-h, Virchow, Virchow2, H-optimus-0, H-optimus-1) and compare their performance against a fully end-to-end trained baseline based on a ResNet50 architecture. We combine FM backbones with representatives of single stage, dual stage and self-attention-based detectors (RetinaNet, Faster R-CNN, Deformable DETR respectively) on the multi-domain MIDOG++ dataset, and on the TUPAC16 dataset as an out-of-domain case. We show that the H-optimus-0 and Virchow models yielded competitive performance, indicating that the latent spaces of current FMs, all trained on image-level self-supervision, are suitable for direct mitotic figure detection and may be slightly more robust on our out-of-domain test case. All code is made available publicly at https://github.com/DeepMicroscopy/FM4MFdet.

Deep learning underlies most modern approaches and tools in computer vision, including biomedical imaging. However, for interactive semantic segmentation (often called pixel classification in this context) and interactive object-level classification (object classification), feature-based shallow learning remains widely used. This is due to the diversity of data in this domain, the lack of large pretraining datasets, and the need for computational and label efficiency. In contrast, state-of-the-art tools for many other vision tasks in microscopy - most notably cellular instance segmentation - already rely on deep learning and have recently benefited substantially from vision foundation models (VFMs), particularly SAM. Here, we investigate whether VFMs can also improve pixel and object classification compared to current approaches. To this end, we evaluate several VFMs, including general-purpose models (SAM, SAM2, SAM3, DINOv3) and domain-specific ones ($μ$SAM, PathoSAM, KRONOS), in combination with shallow learning and attentive probing on five diverse and challenging datasets. Our results demonstrate consistent improvements over hand-crafted features and provide a clear pathway toward practical improvements. Furthermore, our study establishes a benchmark for VFMs in microscopy and informs future developments in this area.

Small-object detection in Unmanned Aerial Vehicle (UAV) imagery requires preserving weak local evidence while using broader context to separate tiny foreground targets from cluttered backgrounds. Existing multi-scale fusion methods improve feature aggregation, but they often add computation or blur fine details during repeated cross-scale fusion. The central challenge is to balance low-SNR target preservation, clutter suppression, and efficient cross-scale context exchange. To address this challenge, we propose the Multi-scale Global-detail Feature Integration Strategy (MGDFIS), a neck-level feature-fusion strategy that couples global context exchange, local-detail recovery, and pixel-level foreground-background recalibration. MGDFIS integrates three coordinated modules: FusionLock-TSS Attention for stabilizing spectral-spatial responses, Global-detail Integration for combining long-range mixing with local detail capture, and Dynamic Pixel Attention for reweighting compact foreground regions. On the controlled VisDrone setting, YOLO26m + MGDFIS improves AP50:95 from 25.7 to 30.2 and AP50 from 37.2 to 44.2 over the YOLO26m baseline, with 96.1 GFLOPs. Additional dataset-specific evaluations report 38.9 AP50 and 21.9 AP50:95 on UAVDT and 97.4 AP50 on CARPK. The code is available at: https://github.com/JackBaixue/MGDFIS.