A Lightweight Fiducial-Based Pipeline for 3D Hyperspectral Mapping of ex-vivo Lumpectomy Specimens

Purpose: 3D reconstruction in minimally invasive surgery (MIS) enables enhanced surgical guidance through improved visualisation, tool tracking, and augmented reality. However, traditional RGB-based keypoint detection and matching pipelines struggle with surgical challenges, such as poor texture and complex illumination. We investigate whether using snapshot hyperspectral imaging (HSI) can provide improved results on keypoint detection and matching surgical scenes. Methods: We developed HyKey, a HYperspectral KEYpoint detection and description model made up of a hybrid 3D-2D convolutional neural network that jointly extracts spatial-spectral features from HSI. The model was trained using synthetic homographic augmentation and epipolar geometry constraints on a robotically-acquired dual-camera RGB-HSI laparoscopic dataset of ex-vivo organs with calibrated camera poses. We benchmarked performance against established RGB-based methods, including SuperPoint and ALIKE. Results: Our HSI-based model outperformed RGB baselines on registered RGB frames, achieving 96.62% mean matching accuracy and 67.18% mean average accuracy at 10 degree on pose estimation, demonstrating consistent improvements across multiple evaluation metrics. Conclusion: Integrating spectral information from an HSI cube offers a promising approach for robust monocular 3D reconstruction in MIS, addressing limitations of texture-poor surgical environments through enhanced spectral-spatial feature discrimination. Our model and dataset are available at https://github.com/alexsaikia/HyKey-Hyperspectral-Keypoint-Detection

Compact snapshot hyperspectral cameras provide rich instantaneous spectral measurements for ground-level machine vision, but at lower spatial resolution than standard RGB cameras. RGB-guided hyperspectral super-resolution (HSR) addresses this limitation by transferring spatial detail from a high-resolution RGB guide to a low-resolution hyperspectral image (HSI). These dual-camera systems are typically in a horizontal rig geometry, requiring cross-camera image alignment due to different fields of view. However, residual misregistration can inject spurious high-frequency details. Existing learned unaligned-fusion methods are usually trained for a fixed spectral support and spatial scale factors and can be computationally demanding, limiting their flexibility across sensors. We propose a lightweight and interpretable RGB-guided HSR framework combining cross-modal flow alignment with model-based Gram-Schmidt orthogonalization fusion. The method first warps the RGB guide onto the HSI grid, then estimates an energy-based confidence weight map by measuring local alignment reliability. This map is then used both in a weighted least-squares spectral regression and in a gated fusion between the super-resolved estimate and an HSI-preserving estimate. Unlike existing learned methods, the proposed framework has a low computational footprint and supports VIS-NIR spectral supports and scale factors without retraining. Experiments on the Real benchmark show that the proposed method improves reconstruction accuracy over learned fusion baselines while remaining substantially faster. On a 34-frame sequence acquired with our real RGB-HSI dual-camera setup, a reduced-resolution quantitative evaluation validates the method under genuine cross-sensor radiometric, noise, and geometric differences, while native-resolution qualitative results demonstrate deployment on the full 51-band VIS-NIR acquisition.

High-resolution images of unprocessed surgical breast tissue can be obtained using microscopy with ultraviolet surface excitation (MUSE). This technique is considered a promising method for checking surgical margins during breast cancer surgery. In this study, MUSE images at 4x and 10x magnifications were compared using patch-level classification methods. Texture analysis (TA) based on local binary patterns (LBP) and deep learning (DL) with a base Vision Transformer (ViT) model were used. Both methods achieved similar performance at both magnifications. Using DL method, both 4x and 10x magnifications achieved 96.30% sensitivity, 100% specificity and 98.18% accuracy. Using TA method, 4x achieved better specificity (100% vs 93.33%) and 10x yielded higher sensitivity (100% vs 93.33%), but both had the same accuracy (96.67%). No clear improvement in performance was observed with 10x magnification. These results show that 4x imaging achieves the same diagnostic accuracy as 10x imaging. At the same time, 4x offers a larger field of view and faster image capture. Therefore, lower magnification can be effectively used in MUSE systems for accurate and efficient intraoperative margin assessment.