Oct 1, 2026, cs.LGJ/K move · Enter open · S save
Tong Chen, Maximilian Holsman, Lin Zhao, Pranam Chatterjee
Department of Computer and Information Science, University of Pennsylvania · Department of Computer Science, Duke University · Department of Bioengineering, University of Pennsylvania
Biomolecular therapeutics often start from known sequences and require targeted editing to improve multiple properties while satisfying hard biochemical and manufacturability constraints. However, existing generative methods do not jointly support multi-objective optimization, hard feasibility, and sequence editing in discrete, variable-length biological spaces. In this work, we introduce Pareto-Constrained Molecule Editing (pCoMole), a framework built on discrete flow matching that steers a pre-trained Edit Flow toward user-specified preferences while enforcing terminal feasibility. pCoMole defines a feasibility-gated terminal distribution using an augmented Tchebycheff utility and realizes the resulting preference tilt through a Doob-h transform of the underlying edit process. To make this construction practical, we approximate the required harmonic function using short Monte Carlo rollouts over candidate edits, yielding an efficient guided editor with provable preference consistency. We validate pCoMole by shrinking GFP while retaining fluorescence-related properties, shortening diverse Cas9 orthologs while preserving PAM specificity, and compressing peptide binders into short peptidomimetics that optimize seven drug-related properties under hard constraints. In wet lab testing, two 229-residue pCoMole-designed eGFP variants retained clear green fluorescence in BL21 cells after 10 deletions, with either one or two substitutions. Together, pCoMole enables constraint-aware, Pareto-aligned editing of biomolecular sequences in discrete, variable-length spaces.