Automatic Field-of-View Adjustment for a View-Expansive Microscope via LSTM-Based Gaze and Pipette Motion Interpretation

Intracytoplasmic sperm injection (ICSI) requires advanced micromanipulation techniques but relies solely on visual feedback and involves frequent interface switching between pipette movement and injector operations. Existing haptic feedback systems primarily focus on pipette puncture forces and do not provide feedback on injector states. We developed an immersive micromanipulation system that unifies operational interfaces and provides McKibben-based haptic sensations to represent aspiration, discharge, and contact between the oocyte and pipette. Users operated both the pipette and injector with a single hand while receiving haptic sensations. A human-participant experiment revealed that the immersive operation interface improved micromanipulation speed and reduced cognitive workload of the micromanipulation compared with conventional methods. Additionally, McKibben-based haptic sensations improved overall system usability. The immersive micromanipulation system with McKibben-based haptic sensations successfully unified operational interfaces and reduced operator workload.

Deep-learning pipelines for microscopy image classification often require expensive, labor- and time-intensive expert annotation to produce high-quality ground truth for training. Recent work has shown that prompt tuning of vision-language models (VLMs) can reduce manual annotation by constructing a small prompt set of expert-verified image-caption exemplars that is reused as few-shot context to classify all remaining images at inference time. To further reduce effort, the VLM can draft captions for candidate exemplars, which experts then verify and lightly edit instead of writing text de novo. However, two practical questions remain unaddressed: (1) which unlabeled images should be prioritized for verification, and (2) how many verified exemplars are needed to reach a performance target. In this work, we address these questions by formulating prompt-set construction as a target-driven active learning problem that prioritizes which images to annotate. We study three complementary selection criteria under strict low-resource constraints with small unlabeled pools. Experiments show that our methods reach the target performance with substantially fewer expert-verified images than random selection, achieving 100% test accuracy with as few as 20 annotated images on average. More broadly, our human-in-the-loop framework demonstrates a human-centered use of generative AI in biomedical image analysis, where experts remain actively involved in verifying and refining model output while significantly reducing annotation cost. Code and data will be publicly available.

Lissajous confocal laser endomicroscopy (CLE) is a promising solution for high speed in vivo optical biopsy for handheld scenarios. However, Lissajous scanning traces a resonant trajectory and samples only the visited pixels per frame; at high frame rates, many pixels remain unvisited, creating structured holes. In this work, we introduce the first benchmark for high-rate Lissajous CLE, consisting of low-quality video clips paired with high-quality reference images. The reference images are wide-FOV mosaics obtained by stitching stabilized, slow-scan frames of the same tissue, enabling temporally aligned supervision. Using this dataset, we propose MIRA, a lightweight recurrent framework for Lissajous CLE restoration that iteratively aggregates temporal context through feature reuse and displacement alignment. Our experiments demonstrate that MIRA outperforms both lightweight and high-complexity baselines in restoration quality while maintaining a favorable computational efficiency suitable for clinical deployment.