Organizations: DST Unit of Nanoscience and Thematic Unit of Excellence, Department of Chemistry, IIT Madras, Chennai 600 036, India. · School of Mechanical Engineering, Faculty of Engineering, Tel Aviv University, Tel-Aviv, Israel. · Faculty of Statistics, Tel Aviv University, Tel-Aviv, Israel. · International Centre for Clean Water, 2nd Floor, B-Block, IIT Madras Research Park, Kanagam Road, Taramani, Chennai 600113, India
Unsafe drinking water remains a major public health concern globally, particularly in low-resource regions where routine microbiological surveillance is limited. Although Escherichia coli is the internationally recognized indicator of fecal contamination, laboratory-based testing is often inaccessible at scale. In this study, we developed and evaluated a two-stage machine-learning framework for predicting E. coli presence in decentralized household point-of-use drinking water in Chennai, India using low-cost physicochemical and contextual indicators. The dataset comprised 3,023 samples collected under the Peoples Water Data initiative; after harmonization, technical cleaning, and outlier screening, 2,207 valid samples were retained. This framework provides a scalable decision-support tool for prioritizing microbiological testing in resource-constrained environments and addresses an important gap in point-of-use contamination risk assessment. Beyond predictive modeling, the present study was conducted within an AI-supported field implementation framework that combined student-facing guidance and real-time QC to improve protocol adherence, traceability, and data reliability in decentralized household water monitoring.
Fecal-contaminated water causes diseases and even death. Current microbial water safety tests require pathogen incubation, taking 24-72 hours and costing $20-$50 per test. This paper presents a solution (DeepScope) exceeding UNICEF's ideal Target Product Profile requirements for presence/absence testing, with an estimated per-test cost of $0.44. By eliminating the need for pathogen incubation, DeepScope reduces testing time by over 98%. In DeepScope, a dataset of microscope images of bacteria and water samples was assembled. An innovative augmentation technique, generating up to 21 trillion images from a single microscope image, was developed. Four convolutional neural network models were developed using transfer learning and regularization techniques, then evaluated on a field-test dataset comprising 100,000 microscope images of unseen, real-world water samples collected from fourteen different water sources across Sammamish, WA. Precision-recall analysis showed the DeepScope model achieves 93% accuracy, with precision of 90% and recall exceeding 94%. The DeepScope model was deployed on a web server, and mobile applications for Android and iOS were developed, enabling Internet-based or smartphone-based water safety testing, with results obtained in seconds.
Early identification of individuals at elevated risk of Chlamydia trachomatis infection may enable optimal use of molecular testing in resource-aware screening. We evaluate the feasibility of pre-test risk stratification (PTRS) using machine-learning models trained on routinely available, non-invasive clinical data. A curated dataset of 93 urine samples with PCR reference labels was analyzed using three feature groups: patient-reported history and symptoms, urine biomarkers from standard urinalysis, and their combination. Five supervised classifiers were evaluated using stratified 5-fold cross-validation with out-of-fold probability estimates. Performance was assessed using area under the receiver operating characteristic curve (AUC) and threshold-dependent metrics, with uncertainty quantified via bootstrap confidence intervals. Models using only patient-reported data showed moderate discrimination (AUC up to 0.72). Urine biomarker-based models demonstrated slightly lower peak discrimination but more consistent performance, with ensemble methods yielding the strongest results. Combining feature groups marginally increased the peak AUC and reduced performance variability across models, indicating improved robustness. Findings indicate that urine biomarkers provide a reliable predictive signal for PTRS that is complementary to patient-reported information, while feature integration enhances robustness. This work supports the integration of non-invasive, routinely available information for PTRS into screening workflows, including decentralized or home-based PCR contexts, to optimize testing prioritization.
Mehrab Mahdian, Marko Lehes, Katrin Krolov +1
Tallinn University of Technology (TalTech), Tallinn, Estonia · Selfdiagnostics Deutschland GmbH, Tallinn, Estonia · Capitol Technology University, Laurel, MD, USA +1
We present FairHealth, an open-source Python library that provides a unified, modular framework for trustworthy machine learning in healthcare applications, with particular focus on low-resource and low-income country (LMIC) settings such as Bangladesh. FairHealth addresses four critical gaps in existing healthcare AI toolkits: (1) the absence of integrated fairness auditing for biosignals and clinical tabular data; (2) the lack of privacy-preserving federated learning tools compatible with standard ML workflows; (3) missing explainability tools tailored for low-bandwidth clinical decision support; and (4) no existing toolkit covering Global South healthcare datasets. Built from five peer-reviewed research contributions, FairHealth provides six modules covering federated learning with homomorphic encryption (fairhealth.federated), intersectional fairness metrics (fairhealth.fairness), hybrid fuzzy-SHAP explainability (fairhealth.explain), multilingual dengue triage (fairhealth.lowresource), equitable disaster aid allocation (fairhealth.equity), and public dataset loaders (fairhealth.datasets). All datasets used are publicly available without institutional data use agreements. FairHealth is installable via pip install fairhealth(PyPI: pypi.org/project/fairhealth/) and available at https://github.com/Farjana-Yesmin/fairhealth.