Instance Segmentation

Current instance segmentation models achieve high performance on average predictions, but lack principled uncertainty quantification: their outputs are not calibrated, and there is no guarantee that a predicted mask is close to the ground truth. To address this limitation, we introduce a conformal prediction algorithm to generate adaptive confidence sets for instance segmentation. Given an image and a pixel coordinate query, our algorithm generates a confidence set of instance predictions for that pixel, with a provable guarantee for the probability that at least one of the predictions has high Intersection-Over-Union (IoU) with the true object instance mask. We apply our algorithm to instance segmentation examples in agricultural field delineation, cell segmentation, and vehicle detection. Empirically, we find that our prediction sets vary in size based on query difficulty and attain the target coverage, outperforming baselines (naive best parameter and morphological dilation-based methods). We provide versions of the algorithm with asymptotic and finite sample guarantees. Our work is the first to capture structural uncertainty in instance segmentation by constructing confidence sets of diverse segmentation predictions.

Background and Objective: Precise and scalable instance segmentation of cell nuclei is a fundamental prerequisite for computational pathology, yet gigapixel whole-slide images (WSIs) pose significant computational challenges. While patch-based processing is standard during training, existing methods are often limited to small tile sizes during inference due to architectural bottlenecks or reliance on computationally expensive post-processing for instance separation. We introduce a faster, scalable, and end-to-end framework capable of processing large-scale image tiles while accurately modeling biologically realistic overlapping nuclei. Methods: We propose LSP-DETR (Local Star Polygon DEtection TRansformer). The model represents nuclei as star-convex polygons and employs a lightweight transformer with linear complexity, enabling the processing of high-resolution images in a single forward pass. A novel radial distance loss accommodates annotation uncertainty, allowing the segmentation of overlapping nuclei to emerge naturally without explicit overlap labels. Results: LSP-DETR achieves state-of-the-art efficiency, with an inference time of 0.45 s/mm^2, a 3.2x speedup over StarDist, the next-fastest method. On PanNuke, the model achieves competitive accuracy (67.5 bPQ), while yielding an F$_1$-score of 0.964 in polygon overlap when evaluated against consensus annotations from two expert pathologists. Furthermore, it outperforms larger models such as LKCell in generalization robustness, reaching an F1-score of 85.0 on MoNuSeg. Conclusions: LSP-DETR bridges the gap between high-fidelity segmentation and practical clinical requirements by eliminating heuristic post-processing. By providing a scalable, linear-complexity solution that naturally handles overlaps between nuclei, this framework sets a new direction for efficient high-throughput WSI analysis in digital pathology.

Interest in forestry automation is growing alongside rapid advances in deep learning. In particular, tree detection and taxonomic classification are seen as core tasks required for automating field surveys and forestry equipment. These operations must often be performed in under-canopy settings, which pose challenging conditions for perception systems, including heavy occlusion, variable lighting, and dense vegetation. Despite this necessity, current work has yet to properly establish the feasibility of simultaneously executing tree detection and taxonomic classification in natural forests, as available datasets primarily focus on urban settings or on a limited number of species. To address this gap, we present SilvaScenes, a benchmark dataset for instance segmentation of tree species from under-canopy images in natural forests. Collected across five bioclimatic domains in Quebec, Canada, our dataset features 1421 trees from 28 species, with segmentation masks for pixel-precise tree trunk detection and fine-grained species annotations from forestry experts. We demonstrate the relevance and difficult nature of SilvaScenes by evaluating modern deep learning approaches, showing that while trunk segmentation is feasible, with a top mean average precision (mAP) of 69.9% and mean average recall (mAR) of 76.4%, species-aware segmentation remains a significant challenge with an mAP and an mAR of only 39.2% and 68.6%, respectively. Alongside additional experiments, we highlight key challenges, namely that species imbalance and tree occlusion figure among the most pressing issues for precise segmentation and identification. Meanwhile, higher image resolutions contribute to significant performance gains and will likely prove fundamental to these tasks moving forward. Our dataset, source code, and models will be made available at https://github.com/norlab-ulaval/SilvaScenes.

Quantitative microstructural characterization is fundamental to materials science, and electron micrographs (EMs) provide indispensable high-resolution insights. However, progress in deep learning-based analysis of EMs has been hampered by the scarcity of large-scale, expert-annotated public datasets. To address this issue, we introduce EM3M, a large-scale and multimodal dataset for instance-level understanding of EMs. EM3M comprises 5,091 high-quality EMs, approximately 3 million instance segmentation annotations, and image-level textual descriptions with disentangled attributes. The dataset is constructed through a rigorous multi-stage curation and validation pipeline, with comprehensive statistical analyses to ensure reliability and reproducibility. Building upon these curated image-text pairs, we further provide a text-to-image diffusion model that serves as a controllable data augmentation engine, demonstrating that synthetic augmentation consistently improves downstream segmentation performance. To establish a systematic benchmark, we evaluate representative instance segmentation methods on EM3M. Our results reveal that conventional detection-based and query-based methods struggle with the extreme instance densities and textural complexities inherent in EMs. We additionally provide an optimized flow-based baseline to facilitate fair comparison and future research. EM3M {Dataset: https://huggingface.co/datasets/UniParser/EM3M}, the generative engine {Generation: https://huggingface.co/UniParser/EM3M-Gen}, and an online demo {Segmentation demo: https://www.bohrium.com/apps/uni-aims} are publicly available to support future research in automated materials analysis.

This study conducted a comprehensive performance evaluation on YOLO11 (or YOLOv11) and YOLOv8, the latest in the "You Only Look Once" (YOLO) series, focusing on their instance segmentation capabilities for immature green apples in orchard environments. YOLO11n-seg achieved the highest mask precision across all categories with a notable score of 0.831, highlighting its effectiveness in fruit detection. YOLO11m-seg and YOLO11l-seg excelled in non-occluded and occluded fruitlet segmentation with scores of 0.851 and 0.829, respectively. Additionally, YOLOv11x-seg led in mask recall for all categories, achieving a score of 0.815, with YOLO11m-seg performing best for non-occluded immature green fruitlets at 0.858 and YOLOv8x-seg leading the occluded category with 0.800. In terms of mean average precision at a 50% intersection over union (mAP@50), YOLOv11m-seg consistently outperformed, registering the highest scores for both box and mask segmentation, at 0.876 and 0.860 for the "All" class and 0.908 and 0.909 for non-occluded immature fruitlets, respectively. YOLO11l-seg and YOLOv8l-seg shared the top box mAP@50 for occluded immature fruitlets at 0.847, while YOLO11m-seg achieved the highest mask mAP@50 of 0.810. Despite the advancements in YOLO11, YOLOv8n surpassed its counterparts in image processing speed, with an impressive inference speed of 3.3 milliseconds, compared to the fastest YOLO11 series model at 4.8 milliseconds, underscoring its suitability for real-time agricultural applications related to complex green fruit environments. Future work will compare YOLO26 (YOLOv26) and YOLO27 (YOLOv27) using the same dataset and training protocol.

Accurate wound classification (WC) and boundary segmentation are essential for guiding clinical decisions in chronic and acute wound management. However, most existing artificial intelligence (AI) models are limited, focusing on a narrow set of wound types, limited variations in wound severity, or a single task (segmentation or classification), which reduces their clinical applicability. This study presents two dedicated instance segmentation models based on You Only Look Once (YOLO)v11 that perform wound boundary segmentation (WBS) and WC across five clinically relevant wound types: burn injury (BI), pressure injury, diabetic foot ulcer, vascular ulcer, and surgical wound. A wound-type balanced dataset of 2,963 annotated images was created to train the models for both tasks, using five-fold cross-validation. Models trained on the original, non-augmented dataset performed consistently across folds, though BI detection accuracy was relatively low; augmenting the dataset with rotation, flipping, and variations in brightness, saturation, and exposure significantly improved performance, particularly for visually subtle BI cases. Among the tested variants, YOLOv11x achieved the best WBS performance (F1-score: 0.9341; mAP50: 0.9629). For WC, YOLOv11m achieved the highest mAP50 (0.9194) and mAP50-95 (0.6950), whereas YOLOv11l achieved the highest F1-score (0.8797). The lightweight YOLOv11n provided comparable accuracy at lower computational cost, making it suitable for resource-constrained deployments. Supported by confusion matrices and visual detection outputs, the results confirm robustness against complex backgrounds and high intra-class variability, demonstrating the potential of YOLOv11-based architectures for accurate, real-time wound analysis in clinical and remote care settings.