Jun 15, 2026, q-bio.QMJ/K move · Enter open · S save
Cora Jostock, Jonas Ort, Henner Koch, Gregor Schiele+1
University of Duisburg-Essen, Intelligent Embedded Systems Lab, Duisburg, Germany · RWTH University Hospital, Department of Neurosurgery, Aachen, Germany · RWTH University Hospital, Department of Epileptology, Aachen, Germany
This paper proposes an unsupervised workflow to pseudo-label extracellular spikes from human brain slice MEA recordings into two putative cell types: pyramidal cells and interneurons. Here, the raw data from the data acquisition system is used and processed. The pipeline for pre-processing includes bandpass filtering, threshold--based spike detection, frame alignment and normalization. In the ML workflow, dimensionality reduction (PCA, t-SNE, UMAP), clustering (GMM, k-means). To achieve an online system, template matching and OSort under varying curation strictness is also considered. All pipelines are evaluated by different cluster quality with within-cluster Pearson correlation, Silhouette score, and Calinski-Harabasz index. Applying stricter curation improves separation at some cost to inclusivity.