3D Plant Phenotyping

A lack of high-throughput phenotyping solutions for root traits in field-grown crops has severely constrained understanding and improvement of below-ground traits and processes. Minirhizotrons are the standard non-destructive root-phenotyping method in field environments. Computer vision solutions are needed to allow automated trait estimation at scale, but training data is scarce and human annotations are often inaccessible because they reside in proprietary software that only exports per-image scalar totals of root length and surface area. Nevertheless, large numeric archives of these root traits already exist. RootQuant showed that the traits can be predicted directly from the whole image by regression, thus removing manually traced masks from the pipeline; RootQuantV2 takes that idea further by replacing RootQuant's CNN backbone with a self-supervised ViT. We adapt a frozen DINOv3 ViT-L/16 with a hybrid parameter-efficient scheme. Training only 11.9M parameters (3.78% of the model), RootQuantV2 achieves length and area $R^2$ of 0.950 and 0.930, respectively, while lowering length/area RMSE by 24.3%/20.7% over RootQuant. RootQuantV2 thus repurposes legacy numeric archives for high-throughput, automated root trait estimation.

Phenotyping an agricultural crop is crucial for studying its entire life cycle, as it provides vital insights to improve yield and, ultimately, food production. Doing the same for crops grown on remote sites is a challenge for the specialists who cannot be available on-site. 3D reconstruction techniques offer a promising solution to this problem by enabling crop digitization, allowing specialists to access the resulting 3D crop models from anywhere at any time. In this work, we evaluate recent 3D reconstruction pipelines for crop phenotyping. We focus on 7 mesh reconstruction pipelines and measure the fidelity and consistency of their outputs qualitatively and quantitatively. Our results suggest that the meshes produced by the GGGS, PGSR, and 2DGS are preferable to the other pipelines, owing to their quantitative metrics and visually pleasing outputs. The GGGS pipeline is better than the second-best pipeline (2DGS) by about 27% on the radar chart with 5 dimensions, namely, User ratings, Chamfer distance, LPIPS, PSNR, and SSIM.

Accurate 3D plant organ segmentation is fundamental to automated phenotyping. Existing approaches rely on annotated training data or species-specific model configurations. We present an annotation-free pipeline for 3D plant organ segmentation, combining text-prompted SAM3 segmentation with semantic neural radiance fields (NeRFs). Given only multi-view RGB images and a list of class names, our zero-shot pipeline produces semantically labeled 3D point clouds without manual annotation, per-species fine-tuning, or domain-specific preprocessing. Multi-view NeRF fusion acts as effective implicit consensus mechanism that lifts imperfect per-frame masks into accurate 3D labels. On a controlled Begonia maculata testbed the SAM3 pipeline achieves 92.6% mIoU, reaching 95.9% of the oracle upper bound established with perfect ground-truth masks. The pipeline was further evaluated on a new dataset spanning ten diverse plant point clouds reaching an average 0.856 mIoU, with leaf and pot IoU above 0.91 and 0.90 for every species, respectively. These results demonstrate that annotation-free 3D plant organ segmentation is now feasible and approaching the range of supervised methods.

Accurate detection of tomato growth stages is essential for stage-specific greenhouse management and precision agriculture. In Bhutan, greenhouse cultivation is affected by altitude variability, large diurnal temperature fluctuations, diffuse illumination, limited automation, and a scarcity of locally annotated datasets, limiting the applicability of conventional deep learning models. This work proposes Pheno-Lite + Efficient Channel Attention (ECA), a lightweight, phenology-aware object detection architecture derived from Ultralytics YOLOv5 for tomato growth stage recognition. A balanced dataset of 2,464 annotated images was constructed from locally collected greenhouse images in Bhutan and publicly available tomato images, with augmentation designed to simulate local greenhouse conditions. The dataset includes vegetative (820), flowering (824), fruiting (820), and background (26) samples. The proposed architecture introduces two customized backbone modules: C3 PhenoLite, which enhances spatial and texture feature extraction using depthwise residual refinement, and C3 ECA, which strengthens inter-channel feature interactions through efficient channel attention. The proposed model achieves 90.6% precision, 88.8% recall, and 92.6% mAP@50, with 4.0 million parameters and 10.9 GFLOPs at 640 x 640 resolution. These results demonstrate its potential for real-time and climate-resilient greenhouse deployment in Bhutan.

In this paper, we introduce a novel framework for 4D plant growth modeling that reconstructs the continuous geometric and topological evolution of plants from sparse temporal observations. Existing methods mainly rely on dense registration, yet reliable dense sequences are hard to obtain due to scanning constraints and self-occlusions, leaving these approaches struggling under large temporal gaps where rapid organ emergence violates local rigidity. To overcome this, we bridge these gaps by formulating plant morphogenesis as a continuous procedural process on a structure-aware Riemannian growth field; this jointly models topology evolution and geometric deformation, preserving botanical hierarchies and stable spatio-temporal correspondences across distant timepoints. Our key idea is to ground symbolic growth rules within a continuous geodesic flow, where organ development follows biologically modulated trajectories that preserve structural coherence under topological changes. We further contribute a 10-day dual-species dataset with dense geometric and semantic annotations. Experiments demonstrate that our method accurately tracks individual organ growth over time and significantly outperforms state-of-the-art baselines in both geometric accuracy and correspondence consistency.

Plant root phenotyping is fundamental to understanding below-ground structures, optimizing crop management, and improving agricultural sustainability. This paper presents a multimodal robotic AI framework that integrates 3D skeleton extraction with language-guided reasoning for interpretable and data-efficient root analysis. We develop an unsupervised skeleton extraction network based on Weighted Laplacian Contraction (W-LBC) to generate high-fidelity structural representations from dense point clouds captured by robotic 3D sensing platforms. Quantitative morphological descriptors, including root count, length, branching angle, and density, are computed from the reconstructed skeleton graph to capture geometric and topological characteristics. Building on these features, we introduce an Evidence-First language modeling framework that fine-tunes GPT as an interactive analytical chatbot using automatically generated instruction--response pairs. Each training sample provides measurable evidence before natural-language reasoning, enabling the model to ground interpretation in quantitative morphology. Through supervised fine-tuning, GPT associates numerical structure with semantic meaning, producing biologically consistent explanations of growth patterns and adaptive traits. Experiments show that the structure-guided framework achieves robust, interpretable reasoning across 12 plant species with diverse root architectures. By integrating unsupervised 3D geometric perception with large-scale language understanding, our approach bridges quantitative analysis and semantic interpretation, establishing a unified paradigm for explainable robotic plant root phenotyping.

Plant biosynthetic gene clusters (BGCs) encode specialized-metabolite pathways, yet curated plant BGC labels remain scarce, hindering supervised discovery at genome scale. Existing plant BGC mining tools are largely signature- and rule-driven and do not fully leverage recent advances in contextual representation learning for modeling long-range domain context and controlling false positives under strong domain shift. We seek an AI-assisted workflow that narrows experimental search space by transferring supervision from well-annotated microbial BGCs to plant genomes. We present PlantBGC, representing genomes as ordered Pfam-domain sequences and learning BGC-likeness with an encoder-only Transformer trained on MIBiG microbial BGCs and adapted to plants via label-free masked language modeling. On microbial benchmarks, PlantBGC achieves token-level AUC = 0.988 (10-fold CV) and 0.979 (leave-class-out). On plants, adaptation improves known-BGC recovery on n = 34 curated loci under strict 100% coverage, increasing recovery from 29.4% to 67.6% and indicating more complete boundaries. GO/KEGG-derived weak supervision reduces proxy primary-like ratio by 48.40% (GO) and 45.20% (KEGG), with consistent per-species reductions (paired Wilcoxon p = 1.53e-5). Compared to plantiSMASH, PlantBGC yields more compact loci on matched regions (median length ratio = 0.278; 93.8% of pairs are shorter).

Semantic segmentation in agricultural imagery is often evaluated under in-domain protocols, yet practical deployment requires robustness to appearance perturbations, limited annotations, and cross domain shift. This paper presents a diffusion-guided hybrid segmentation framework in which U-Net, DeepLabV3+, and SegFormer backbones generate coarse masks that are refined by Denoising Diffusion Probabilistic Models (DDPM), latent diffusion, or semantic-guided diffusion. The framework is evaluated through a 3x3 architectural screening study on PlantSegV3, followed by boundary-constrained optimization, perturbation-guided retraining, low-data evaluation, constrained hyperparameter screening, and controlled cross-domain adaptation. On PlantSegV3, the best selected hybrid model achieves 71.83% refined mean Intersection-over-Union (mIoU) and 26.10% refined Boundary-F1, and the selected models remain stable under substantially reduced supervision, demonstrating strong annotation efficiency. Perturbation analysis identifies grayscale conversion, fog, coarse dropout, and shadow as the most disruptive appearance shifts, and the resulting augmentation policy substantially improves robustness during retraining. The adapted models further show effective transfer to external agricultural datasets under limited target supervision, indicating that diffusion refinement and boundary-aware optimization provide transferable structural priors. Overall, the results show that carefully matched backbone-refiner pairings, combined with perturbation-aware retraining, can improve structural delineation and robustness under realistic resource and distribution constraints.

Leaf veins exhibit remarkable diversity in architecture and patterning, yet existing gene--environment association studies have primarily quantified leaf venation using a small collection of low-dimensional summary traits, thereby discarding most of the structural information contained in the original images. We propose an integrated deep learning and statistical framework. The proposed framework achieves four methodological advances. First, it represents the complete leaf vascular architecture as a whole-network image phenotype. Second, it fine-tunes the deep learning-based Edge Detection with Transformers (EDTER) model to accurately extract whole-network leaf vascular architecture from RGB images by jointly learning local and global contextual features. Third, it constructs a new annotated leaf image database by integrating edge maps generated by DiffusionEdge with the Berkeley Segmentation Database (BSDS500). Fourth, it applies Semiparametric Sparse Canonical Correlation Analysis (SSCCA) to perform variable selection and model associations between repeatedly measured high-dimensional Bivariate image responses and high-dimensional predictors while simultaneously accommodating sparse, zero-inflated data represented by edge maps through a truncated latent Gaussian copula model. Two simulation studies demonstrate the performance of the proposed framework under increasing levels of complexity. Application to a real \emph{Populus} dataset identifies three significant gene--geography interactions associated with leaf vascular architecture, providing new biological insights and establishing a broadly applicable methodological framework for high-dimensional complex image phenotypes.

Vision-based automation is an excellent candidate for reducing manual labor in greenhouse crop production and phenotyping. However, progress is constrained by the lack of annotated training data. Recent advances in vision-based foundational models have shown promising results in zero-shot generalization to novel domains, but their performance drops in complex agricultural environments. In this work, we present a sim-to-real framework for tomato plant segmentation that combines synthetic data generation with fine-tuning of a foundation model. We model a commercial cherry tomato greenhouse and use it to generate a large-scale synthetic dataset under diverse viewpoints, lighting conditions, and plant morphology. Subsequently, we fine-tune the Segment Anything Model 3 (SAM 3) on the synthetic dataset, specializing its text-conditioned segmentation behavior for greenhouse crop organs while retaining the general visual prior that makes zero-shot transfer possible. By evaluating our framework on multiple real-world greenhouse datasets, we demonstrate that combining synthetic data with SAM 3 fine-tuning significantly improves segmentation performance and model confidence. To support community benchmarking, we publicly release the procedural model, the generated synthetic dataset, and our fine-tuned SAM 3 weights.

Quantifying plant growth dynamics from sparse longitudinal 3D observations is fundamental for agriculture and plant sciences. Yet, plants pose unique challenges: they undergo intricate non-rigid deformations, exhibit changing topology as new organs emerge, and often lack explicit temporal correspondences between consecutive data acquisitions due to newly formed tissue. Methods designed for general scenes struggle to model topology changes and asynchronous organ growth characteristic of plants. To address these challenges, we introduce GrowFields, a compositional dynamic neural field representation for organ-aware 4D plant growth modelling from point cloud time series. Our approach decomposes a plant into its constituent organs and aligns each organ into its own canonical coordinate frame, isolating intrinsic growth patterns from global plant motion. We then learn a shared continuous neural deformation field that models temporal dynamics across all organs, conditioned on learnable per-organ latent codes capturing organ identity and growth characteristics. The resulting modular yet unified representation naturally accommodates the asynchronous development of plant organs while remaining grounded in the practical setting of organ-level plant tracking. We evaluate GrowFields on growth sequences from four plant species, assessing geometric fitting and organ tracking accuracy using manually annotated leaf-tip trajectories. Results demonstrate consistent improvements in spatial precision, temporal coherence, and morphological fidelity over a range of existing representations.

High-throughput plant phenotyping generates valuable data that often remains trapped in unstructured text and isolated RGB images. To bridge this semantic gap, we propose a framework for constructing a multimodal granular Knowledge Graph (KG) to monitor genotype-phenotype interactions across time and experiments. In this work, we focus on wheat Triticum aestivum as a representative target crop to validate our methodology across complex canopy environments. Our pipeline first distills noisy field notes to extract entities and relations, dynamically constructing the KG by converting unique instances into hierarchical class entities via RDF-typing. These graph nodes are then aligned with standardized ontologies (PO, RO, WTO) using PlantDeBERTa. To visually ground the constructed graph, a Vision-Language Model paired with a wheat-segmentation ViT generates attention-based softmaps, linking specific KG entities directly to image pixels. We introduce a central observation node Plant_Obs_Id to connect these multimodal subgraphs temporally. Evaluated on 500 curated WisWheat samples using Pointing Game accuracy, Visual Word Sense Disambiguation (VWSD), and rank-based metrics, our neuro-symbolic approach successfully maps complex field observations to a structured graph. This enables automated field note auditing, temporal stress monitoring, and precise spatial trait localization for wheat breeders.

3D plant phenotyping is notoriously known to be procedure-complicated and of low throughput due to the extensive multi-view imaging, the fragile 3D reconstruction pipeline, and the additional cost from reconstructed geometry to phenotypic extraction. These limitations are further amplified in low-cost data acquisition, where smartphone videos or sparsely sampled multi-view images provide limited view overlap and self-occlusion. In this work, we show that the conventional 3D plant phenotyping pipeline could be streamlined and significantly accelerated with 3D Foundation Models (3DFMs), and particularly, present one of the first cross-crop 3D phenotyping frameworks powered by 3DFMs. The framework replaces COLMAP-style sparse initialization with 3DFM-based feed-forward geometric recovery, combines geometry-constrained 3D Gaussian Splatting for dense reconstruction, enables few-view reconstruction through iterative view synthesis and refinement, and converts reconstructed geometry into measurable organs through 2D-to-3D semantic transfer, metric scale recovery, and organ instance separation. We further construct a cross-crop dataset with smartphone-based image acquisition, diverse plant morphologies, and manual annotations for segmentation and phenotypic evaluation. Experiments across 26 plant sequences show that 3D Foundation Models reduce the average reconstruction time from 6.52 minutes to 1.58 seconds while maintaining high reconstruction quality and phenotyping accuracy. These results suggest a fresh technical route for high-throughput 3D plant phenotyping, from low-cost image acquisition to fast reconstruction, perception, scale recovery, and phenotypic measurement.

High-throughput plant phenotyping now generates image derived datasets far faster than scientists can analyze them. At Oak Ridge National Laboratory's Advanced Plant Phenotyping Laboratory (APPL), automated stations image hundreds of plants daily across multiple remote sensing modalities; yet, trait extraction and interpretation remain manual, expert-bound, and strictly post-hoc, making analysis, not acquisition, the binding constraint on discovery. We present an end-to-end agentic AI framework that turns the facility from a data factory into an interactive autonomous, discovery platform, where scientists partner with AI agents to accelerate time to insight. A conversational Co-Scientist Agent translates a scientist's natural-language question into a structured analysis plan, and a headless Compute Agent dispatches Vision Transformer segmentation and trait extraction on the Frontier exascale supercomputer. The two agents run in separate security and resource domains and communicate over a secure, token-authenticated streaming channel, a design that accounts for the federation, data-movement, and provenance realities cloud-native agentic frameworks ignore, ensuring end-to-end provenance is captured for every interaction. The framework turns a days- to weeks-long analysis process into an interactive loop where agents reason over results, recommend next analyses, and respond to follow-up questions in seconds.

Understanding which genes control which traits in an organism remains one of the central challenges in biology. Despite significant advances in data collection technology, our ability to map genes to traits is still limited. This genome-to-phenome (G2P) challenge spans several problem domains, including plant breeding, and requires methods capable of reasoning over high-dimensional, heterogeneous, and biologically structured data. Current datasets and data repositories, however, are not well-equipped for this task. Current studies do not link gene expression and trait data, and most focus on very specific traits, limiting the breadth of possible correlations. To address this gap, we present the novel Gene-Graph Regression for Arabidopsis Functional Traits (GRAFT) dataset, a curated multi-modal dataset linking gene expression profiles with phenotypic trait measurements in Arabidopsis thaliana, a model organism in plant biology. GRAFT supports tasks such as phenotype prediction and interpretable graph learning. In addition, we benchmark conventional regression and explanatory baselines, including a biologically-informed hypergraph baseline, to validate gene-trait associations. To the best of our knowledge, this is the first dataset to provide multimodal gene information and heterogeneous trait or phenotype data for the same Arabidopsis thaliana specimens. With GRAFT, we aim to foster research to accurately understand the relationship between genotypes and phenotypes using gene information, higher-order gene pairings, and trait data from multiple sources.

Morphological traits provide important evidence for phylogenetic reconstruction and evolutionary relationship analysis. Recent image-based approaches have introduced deep learning, particularly convolutional models, to derive morphological features from specimen images, but these methods generally rely on single-modality visual representations and do not explicitly incorporate morphological semantics. This study proposes a morphology-aware multimodal alignment framework for insect phylogenetic reconstruction. The framework combines specimen images with curated morphological descriptions by adapting a vision transformer through parameter-efficient fine-tuning and supervised contrastive learning, followed by image-text alignment in a shared latent space. The learned image embeddings are then used as continuous traits for Bayesian phylogenetic reconstruction. On the public Rove-Tree-11 dataset, comparative and ablation experiments across multiple visual backbones and feature adaptation strategies demonstrate that multimodal alignment improves topological agreement with the reference phylogeny. The results indicate that the proposed framework can derive morphology-aware visual traits for computational phylogenetic reconstruction.

Accurate estimation of wheat spike volume is important for yield component analysis and stress resilience assessment, yet field-based measurement remains challenging. Active 3D sensing methods such as Light Detection and Ranging (LiDAR) or time-of-flight (ToF) are sensitive to plant motion or poorly suited to outdoor conditions, while 3D reconstructions are computationally expensive. Direct 2D image processing would offer computational advantages, but image-based models lack explicit geometric information. We therefore propose a hybrid 2D-3D approach with knowledge distillation during training while enabling efficient image-only inference. First, we train a rigid-invariant point cloud network using distance-based histogram features to obtain pose-robust geometric representations. We then combine the 3D model with a proposed multi-view image-based regulated Transformer (RT) in an ensemble architecture. Finally, we distill the ensemble knowledge into a purely image-based student model using either feature-based or label-based distillation. The two distilled RTs reduce the mean absolute error (MAE) from 654.31 mm$^3$ of the non-distilled RT to 639.93 mm$^3$ and 644.62 mm$^3$, and increase correlation from 0.76 to 0.77 and 0.82, respectively. At the same time, inference time is reduced from 160 ms to 1.4 ms per spike. Distillation further mitigates volume-dependent bias and reshapes the latent representation of the image model toward a geometry-aware shape. Our results demonstrate that 3D-informed training of a 2D Transformer allows for scalable and efficient spike volume estimation for high-throughput field phenotyping.

High-dimensional biological data often exhibit a severe mismatch between feature dimensionality and sample size, making reliable classification difficult in extremely small-data regimes. In these settings, kernel methods can lose discriminative power when latent compression fails to preserve class-separating structure. We study this problem in fine-grained plant phenomics and propose a hybrid workflow that compresses 1280-dimensional deep image embeddings into a 64-dimensional PCA space and then restructures them into an 11-dimensional supervised latent space using Linear Discriminant Analysis (LDA), followed by GPU-accelerated Quantum Kernel Alignment (QKA) on NVIDIA L40S hardware. Empirically, supervised latent restructuring substantially improves the geometric separability of the compressed representation, increasing the Silhouette coefficient from 0.003 in the raw embedding space and -0.006 in PCA-64 to 0.197 in the supervised LDA-11 space. However, downstream classical evaluation reveals a clear compression trade-off: Linear SVM and XGBoost improve in the restructured latent space, whereas RBF-SVM and Random Forest degrade under the same 11-dimensional bottleneck. Under a constrained optimization budget, QKA in this regime remains challenging, indicating that latent geometry alone is not sufficient for strong trainable quantum performance. These findings position representation geometry as a central design variable in small-data quantum learning and expose the practical difficulty of recovering nonlinear discriminative structure from aggressively compressed biological representations.

Accurate estimation of plant skeletal structures (e.g., branching structures) from images is essential for smart agriculture and plant science. Unlike human skeletons with fixed topology, plant skeleton estimation presents a unique challenge, i.e., estimating arbitrary tree graphs from images. To address this problem, we introduce PlantPose, a universal plant skeleton estimator via tree-constrained graph generation. PlantPose combines learning-based graph generation with traditional graph algorithms to enforce tree constraints during the training loop. To enhance the model's generalization capability, we curate a large and diverse dataset comprising real-world and synthetic plant images, along with simplified representations (e.g., sketches and abstract drawings). This dataset enables the generalized model to adapt to diverse input styles and categories of plant images while preserving topological consistency. Our approach demonstrates robust and accurate plant skeleton estimation across multiple domains, including previously unseen out-of-domain scenarios. Further analyses highlight the method's strengths and limitations in handling complex, heterogeneous data distributions. All implementations and datasets are available at https://github.com/huntorochi/PlantPose/.

Cell-type-specific marker genes are fundamental to plant biology, yet existing resources primarily rely on curated databases or high-throughput studies without explicitly modeling the supporting evidence found in scientific literature. We introduce PlantMarkerBench, a multi-species benchmark for evaluating literature-grounded plant marker evidence interpretation from full-text biological papers. PlantMarkerBench is constructed using a modular curation pipeline integrating large-scale literature retrieval, hybrid search, species-aware biological grounding, structured evidence extraction, and targeted human review. The benchmark spans four plant species -- Arabidopsis, maize, rice, and tomato -- and contains 5,550 sentence-level evidence instances annotated for marker-evidence validity, evidence type, and support strength. We define two benchmark tasks: determining whether a candidate sentence provides valid marker evidence for a gene-cell-type pair, and classifying the evidence into expression, localization, function, indirect, or negative categories. We benchmark diverse open-weight and closed-source language models across species and prompting strategies. Although frontier models achieve relatively strong performance on direct expression evidence, performance drops substantially on functional, indirect, and weak-support evidence, with evidence-type confusion emerging as a dominant failure mode. Open-weight models additionally exhibit elevated false-positive rates under ambiguous biological contexts. PlantMarkerBench provides a challenging and reproducible evaluation framework for literature-grounded biological evidence attribution and supports future research on trustworthy scientific information extraction and AI-assisted plant biology.

High-throughput plant phenotyping, the quantitative measurement of observable plant traits, is critical for modern breeding but remains constrained by a "phenotyping bottleneck," where manual data collection is labor-intensive and prone to observer bias. Conventional closed-set computer vision systems fail to address this challenge, as they require extensive species-specific annotation and lack the flexibility to handle diverse breeding populations. To bridge this gap, we present CropVLM, a Vision-Language Model (VLM) adapted for the agricultural domain via Domain-Specific Semantic Alignment (DSSA). Trained on 52,987 manually selected image-caption pairs covering 37 species in natural field conditions, CropVLM effectively maps agronomic terminology to fine-grained visual features. We further introduce the Hybrid Open-Set Localization Network (HOS-Net), an architecture that integrates CropVLM to enable the detection of novel crops solely from natural language descriptions without retraining. By eliminating the reliance on species-specific training data, CropVLM provides a scalable solution for high-throughput phenotyping, accelerating genetic gain and facilitating large-scale biodiversity research essential for sustainable agriculture. The trained model weights and complete pipeline implementation are publicly available at: https://github.com/boudiafA/CropVLM. In comprehensive evaluations, CropVLM achieves 72.51% zero-shot classification accuracy, outperforming seven CLIP-style baselines. Our detection pipeline demonstrates superior zero-shot generalization to novel species, achieving 49.17 AP50 on our CVTCropDet benchmark and 50.73 AP50 on tropical fruit species, compared to 34.89 and 48.58 for the next-best method, respectively.

Plant phenology-the study of recurrent life cycle events-is essential for understanding ecosystem dynamics and their responses to climate change impacts. While Unmanned Aerial Vehicles (UAVs) and near-surface cameras enable high-resolution monitoring, identifying plant species across time remains computationally challenging. State-of-the-art approaches, specifically Multi-Temporal Convolutional Networks (CNNs), rely on rigid multi-branch architectures that scale poorly with longer time series and require large spatial context windows. In this paper, we present an extensive study on optimizing Vision Transformers (ViTs) for efficient spatio-temporal vegetation pixel classification. We conducted a comprehensive ablation study analyzing seven key design dimensions, including: (i) data normalization; (ii) spectral arrangement; (iii) boundary handling; (iv) spatial context window shape and size; (v) tokenization strategies; (vi) positional encoding; and (vii) feature aggregation strategies. Our method was evaluated on two datasets from the Brazilian Cerrado biome, Serra do Cipó (aerial imagery) and Itirapina (near-surface imagery). Experimental results demonstrate that our ViT approach offers a substantial improvement in computational efficiency while maintaining competitive classification performance. Notably, our ViT reduces Floating Point Operations (FLOPs) by an order of magnitude and maintains constant parameter complexity regardless of the time series length, whereas the CNN baseline scales linearly. Our findings confirm that ViTs are a robust, scalable solution for resource-constrained phenological monitoring systems.

Stomata play a crucial role in regulating plant physiological processes and reflecting environmental responses. However, accurate and high-throughput stomatal phenotyping remains challenging, as conventional approaches rely on destructive sampling and manual annotation, restricting large-scale and field deployment. To overcome these limitations, a noninvasive restoration-detection integrated framework, termed StomaD2, is developed to achieve accurate and fast stomatal phenotyping under complex imaging conditions. The framework incorporates a diffusion-based restoration module to recover degraded images and a specialized rotated object detection network tailored to the small, dense, and cluttered characteristics of stomata. The proposed network enhances feature representation through three key innovations: a column-wise structure for global feature interaction, context-aware resampling and reweighting mechanism to improve multi-scale consistency, and a feature reassembly module to boost discrimination against complex backgrounds. In extensive comparisons, StomaD2 demonstrated state-of-the-art performance. On public Maize and Wheat datasets, it achieved accuracies of 0.994 and 0.992, respectively, significantly outperforming existing benchmarks. When benchmarked against ten other advanced models, including Oriented Former and YOLOv12, StomaD2 achieved a top-tier F1-score/mAP of 0.989. The framework is integrated into a user-friendly, field-operable system that supports the fast extraction of eight stomatal phenotypes, such as density and conductance. Validated on more than 130 plant species, StomaD2's results highlight its strong generalizability and potential for large-scale phenotyping, plant physiology analysis, and precision agriculture applications.